LitCovid-sentences  

PMC:7091888 / 13135-15676 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T73 0-215 Sentence denotes Like SARS-CoV and MERS-CoV, SARS-CoV-2 also originates from bats.22–24 Next, we detected the binding affinity of the identified SARS-CoV-2 RBD to bat ACE2 (bACE2) and compared this binding with that of SARS-CoV RBD.
T74 216-399 Sentence denotes We transiently transfected a bACE2-expressing plasmid into 293T cells and included a hACE2-expressing plasmid as a control, followed by detection of fluorescence intensity 48 h later.
T75 400-632 Sentence denotes Results indicated that SARS-CoV-2 RBD bound strongly to 293T-expressed bACE2 with intensity similar to that of its binding to 293T-expressed hACE2 (Fig. 3a, c), and that this binding occurred in a dose-dependent manner (Fig. 3e, f).
T76 633-721 Sentence denotes In addition, the binding affinity between SARS-CoV-2 RBD and 293T-expressed bACE2 (EC50:
T77 722-749 Sentence denotes 0.08 μg/ml) or hACE2 (EC50:
T78 750-849 Sentence denotes 0.14 μg/ml) was significantly higher than that between SARS-CoV RBD and 293T-expressed bACE2 (EC50:
T79 850-877 Sentence denotes 0.96 μg/ml) or hACE2 (EC50:
T80 878-905 Sentence denotes 1.32 μg/ml) (Fig. 3b, d–f).
T81 906-995 Sentence denotes Nevertheless, MERS-CoV RBD bound neither bACE2- nor hACE2-expressing 293T cells (Fig. 3).
T82 996-1196 Sentence denotes These data suggest that SARS-CoV-2 RBD can bind to both bACE2 and hACE2 with significantly stronger binding than that of SARS-CoV RBD to either bACE2 or hACE2, supporting the bat origin of SARS-CoV-2.
T83 1197-1284 Sentence denotes These results may partially explain why SARS-CoV-2 is more transmissible than SARS-CoV.
T84 1285-1369 Sentence denotes Fig. 3 Comparison of SARS-CoV-2 RBD protein binding to human and bat ACE2 receptors.
T85 1370-1512 Sentence denotes Flow cytometry analysis of SARS-CoV-2 RBD binding to hACE2 and bat ACE2 (bACE2) receptors in 293T cells transiently expressing hACE2 or bACE2.
T86 1513-1662 Sentence denotes 293T cells were transiently transfected with hACE2 or bACE2 plasmid and incubated with SARS-CoV-2 RBD protein at various concentrations for analysis.
T87 1663-1724 Sentence denotes SARS-CoV RBD and MERS-CoV RBD proteins were used as controls.
T88 1725-1872 Sentence denotes Representative images of SARS-CoV-2 RBD protein (2.5 μg/ml) binding to bACE2/293T (a, black line), or hACE2/293T (c, black line), cells were shown.
T89 1873-2007 Sentence denotes Binding of SARS-CoV RBD protein (2.5 μg/ml) to bACE2/293T (b, red line), or hACE2/293T (d, red line), cells were used as a comparison.
T90 2008-2240 Sentence denotes MERS-CoV RBD protein (green line) and mock-incubated (gray shading) cells (a–d) were included as controls. e, f Dose-dependent binding of SARS-CoV-2 RBD protein to bACE2/293T (e), or hACE2/293T (f), cells by flow cytometry analysis.
T91 2241-2432 Sentence denotes Significant differences between binding of SARS-CoV-2 RBD (black) and SARS-CoV RBD (red) to cell-associated bACE2 receptor (e), or hACE2 receptor (f) were identified based on the EC50 values.
T92 2433-2481 Sentence denotes The data are presented as mean ± s.e.m. (n = 4).
T93 2482-2541 Sentence denotes Experiments were repeated twice and yielded similar results