LitCovid-sentences  

PMC:7075884 / 7270-8817 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
T49 0-121 Sentence denotes To further assess the effect of GA on plaque formation, we used the CMVPT30-GFP strain, which produces cell-free virions.
T50 122-279 Sentence denotes HFF monolayers in 24-well plates pretreated with GA (1–20 µM) for 1 h were inoculated with 50–100 plaque-forming units of CMVPT30-GFP per well.16, (Fig. 1B).
T51 280-356 Sentence denotes The number of plaques were counted after 7 days and the IC50 was determined.
T52 357-457 Sentence denotes As predicted by the previously-determined IC50, at 5 and at 10 µM the antiviral effect was apparent.
T53 458-575 Sentence denotes Furthermore, at 10 µM, only single cells were infected, indicating that there was no cell-to-cell virus transmission.
T54 576-687 Sentence denotes These results may be explained as inhibition of secondary infections or that viral entry may be a target of GA.
T55 688-882 Sentence denotes To verify these results, we compared the inhibition of infection by GA using a plaque reduction assay (PRA) similar to the PRA for ganciclovir (GCV), the preferred antiviral HCMV drug of choice.
T56 883-1107 Sentence denotes The results showed that although there was strong inhibition of the virus at 16 µM GCV, there were still visible plaques (>5 adjacent cells showing HCMV cytopathic effect) indicating cell-to-cell transmission of the virions.
T57 1108-1309 Sentence denotes On the contrary, CMVPT30-GFP infected monolayers that were treated with 10 µM GA only displayed individual infected cells in intact HFF monolayers, (Fig. 1C), thus indicating no cell-cell transmission.
T58 1310-1347 Sentence denotes We also tested GA C17:1 and GA C13:0.
T59 1348-1455 Sentence denotes Both GA compounds had a strong inhibitory effect on HCMV infection by PRA, similar to GA C15:1 (Fig. 1D,E).
T60 1456-1547 Sentence denotes None of the GA structures showed cytotoxicity at their active concentrations, respectively.