LitCovid-sentences  

PMC:7075884 / 39579-41138 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T290 0-171 Sentence denotes HFF were grown in 24 well plates to 100% confluency in MEM with 10% FBS to establish viral inhibition of two low-passage clinical strains (BI-6 and CH19) via plaque assay.
T291 172-331 Sentence denotes Cells infected with low-passage HCMV clinical strains (CH19 and BI6) do not release extracellular virus, but they display typical HCMV cytopathic effect (CPE).
T292 332-406 Sentence denotes The percentage of infected cells was estimated visually in HFF monolayers.
T293 407-496 Sentence denotes Dilutions of trypsinized cells were used as inocula for HFF monolayers in 24 well plates.
T294 497-624 Sentence denotes After 7 days in culture, plaques were counted to determine the average number of plaques in 4-well replicates of each dilution.
T295 625-714 Sentence denotes The optimal dilution for each virus strain produced an average of 60–80 plaques per well.
T296 715-791 Sentence denotes CMVPT30-GFP having undergone multiple passages produces extracellular virus.
T297 792-875 Sentence denotes Viral infection was quantitatively determined by PRA of culture supernatant medium.
T298 876-1070 Sentence denotes Cells were either exposed to GA for three hours prior to inoculation, or were inoculated prior to addition of medium containing GA at concentrations of 1, 2, 5, 10, and 20 µM in MEM with 1% FBS.
T299 1071-1136 Sentence denotes One set of four wells was treated with DMSO as a vehicle control.
T300 1137-1226 Sentence denotes For each experiment the remaining concentrations were represented in quadruplicate wells.
T301 1227-1416 Sentence denotes Plates were read beginning at 7 days post inoculation (dpi) to determine the GA concentration producing a reduction of 50% in the number of plaques relative to vehicle control wells (IC50).
T302 1417-1559 Sentence denotes Data were normalized to the vehicle controls, and probit analysis in SPSS was used to obtain 95% confidence intervals of the mean IC50 values.