| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T240 |
0-21 |
Sentence |
denotes |
Western blot analyses |
| T241 |
22-234 |
Sentence |
denotes |
For HSV-1, cells were harvested at the indicated times, collected by low-speed centrifugation and rinsed in PBS, then resuspended in RIPA lysis buffer and protease inhibitors (Complete Protease Inhibitor; Roche). |
| T242 |
235-311 |
Sentence |
denotes |
Approximately 60 μg of protein per sample was subjected to further analysis. |
| T243 |
312-522 |
Sentence |
denotes |
Proteins were electrophoretically separated on 8 to 10% denaturing polyacrylamide gels, electrically transferred to nitrocellulose sheets, blocked by 5% BSA in Tween PBS and reacted with the primary antibodies. |
| T244 |
523-680 |
Sentence |
denotes |
The mouse monoclonal antibody to US11 (Goodwin Institute for Cancer Research), and the mouse monoclonal antibody to ICP8, were used at a dilution of 1:1,000. |
| T245 |
681-752 |
Sentence |
denotes |
The mouse monoclonal antibody to ICP27 was used at a dilution of 1:250. |
| T246 |
753-833 |
Sentence |
denotes |
The mouse monoclonal antibody to β-actin (Sigma) was used at a 1:5,000 dilution. |
| T247 |
834-973 |
Sentence |
denotes |
Membranes were then reacted with the appropriate secondary antibody conjugated either to alkaline phosphatase or to horseradish peroxidase. |
| T248 |
974-1206 |
Sentence |
denotes |
Protein bands were visualized with 5-bromo-4-chloro-3-indolylphosphate–nitroblue tetrazolium (Denville Scientific, Inc.) or with ECL Western Blot detection reagents (Amersham Biosciences) according to the manufacturer’s instruction. |
