LitCovid-sentences  

PMC:7075884 / 30265-33179 JSONTXT 12 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T215 0-21 Sentence denotes Cells, viruses and GA
T216 22-207 Sentence denotes HEp2 cells, obtained from the American Type Culture Collection (ATCC Rockville, MD), were grown in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 5% fetal bovine serum (FBS).
T217 208-300 Sentence denotes HEK293T/17 (293T) cells obtained from the ATCC were grown in DMEM supplemented with 10% FBS.
T218 301-394 Sentence denotes HSV-1 strain F, a limited-passage isolate, is the prototype strain used in this laboratory28.
T219 395-462 Sentence denotes GFP-HSV-1 (strain 17), was a generous gift from Dr. Peter O’Hare29.
T220 463-652 Sentence denotes Vero cells obtained from the ATCC were cultured in complete DMEM (cDMEM) containing 10% FBS, 2 mM L-glutamine, 100 U/mL penicillin/streptomycin, and 10 mM Hepes buffer at 37 °C with 5% CO2.
T221 653-917 Sentence denotes Fetal-derived Normal Human Astrocytes (NHA, Lonza) were maintained in Astrocyte Growth Medium (AGM, Lonza) supplemented with 0.3% FBS, 30 µl/mL ascorbic acid, 1 µl/mL rhEGF, 30 µg/mL gentamicin, 15 μg/mL amphotericin B, 2.5 µl/mL insulin, and 10 µl/mL L-glutamine.
T222 918-970 Sentence denotes Early passages (1–4) were used in these experiments.
T223 971-1069 Sentence denotes HCMV studies were performed using cell-associated low-passage clinical isolates CH1914 and BI-615.
T224 1070-1179 Sentence denotes CH19 is resistant to the first-line HCMV antiviral drug, ganciclovir (GCV), while BI-6 is susceptible to GCV.
T225 1180-1314 Sentence denotes Additional experiments were performed using CMVPT30-GFP16, which expresses green fluorescent protein and produces extracellular virus.
T226 1315-1537 Sentence denotes ZIKV strain PRVABC59 obtained from the American Type Culture Collection (ATCC; Manassas, VA) was propagated in Vero cells grown in T-150 flasks by infection at 1:50 dilution of viral stock (MOI 0.25) in the absence of FBS.
T227 1538-1616 Sentence denotes The medium was removed and replaced 6 h post-infection (hpi) with fresh cDMEM.
T228 1617-1778 Sentence denotes Supernatant was collected at 72 hpi, clarified by centrifugation at 350 × g for 5 min, and filtered through a 0.45-μm surfactant-free cellulose acetate membrane.
T229 1779-1913 Sentence denotes For mock infections, supernatant was collected from uninfected Vero cells and prepared by the same protocol used to make virus stocks.
T230 1914-1947 Sentence denotes Virus was titered by focus assay.
T231 1948-2322 Sentence denotes Briefly, infected Vero cells, 24 hpi were fixed and permeabilized using Cytofix/Cytoperm Solution Kit (BD Biosciences) according to the manufacturer’s instructions and stained with a mouse monoclonal antibody (mAb) specific for flavivirus group envelope proteins (1:250; EMD Millipore; clone D1–4G2-4-15) followed by incubation with an anti-mouse IgG - PE (1:1000 dilution).
T232 2323-2483 Sentence denotes Samples were run through an LSR II flow cytometer and data were collected with FACSDiva software (BD Biosciences) and analyzed using FlowJo Software (TreeStar).
T233 2484-2521 Sentence denotes Human Adenovirus Type5 (dE1/E3), Cat.
T234 2522-2526 Sentence denotes No.:
T235 2527-2567 Sentence denotes 1060, was purchased from Vector Biolabs.
T236 2568-2645 Sentence denotes GA C13:0, C15:1, and C17:1, were purchased from Sigma Aldrich, St. Louis, MO.
T237 2646-2709 Sentence denotes GA was diluted in methanol or DMSO to a concentration of 50 mM.
T238 2710-2839 Sentence denotes Effects of GA in all assays and models used in this study have been compared to vehicle-treated (i.e. methanol or DMSO) controls.
T239 2840-2914 Sentence denotes Cells or viral stocks were treated with the indicated concentration of GA.