LitCovid-sentences  

PMC:7075884 / 21579-24326 JSONTXT 10 Projects

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Id Subject Object Predicate Lexical cue
T155 0-96 Sentence denotes GA inhibition of enveloped viruses correlates with inhibition of viral DNA and protein synthesis
T156 97-246 Sentence denotes To validate the PRA observations, we tested whether there is an effect on downstream viral DNA synthesis in HCMV infected cells when treated with GA.
T157 247-363 Sentence denotes HFF were inoculated with HCMV for 3 hours, then washed and incubated with different concentrations of GA or vehicle.
T158 364-499 Sentence denotes DNA was extracted from 4 pooled wells of HFF at 7 days post inoculation, and then analyzed by qPCR targeting the viral polymerase gene.
T159 500-601 Sentence denotes DNA copies were reduced in a dose dependent manner by the addition of GA to culture medium (Fig. 7A).
T160 602-755 Sentence denotes Although HCMV DNA copy numbers were reduced by GA, this could be secondary to inhibition of virus entry rather than direct inhibition of DNA replication.
T161 756-902 Sentence denotes Figure 7 GA inhibits HSV-1 replication by inhibition of viral protein synthesis and HCMV by DNA synthesis. (A) Effect of GA on HCMV DNA synthesis.
T162 903-1030 Sentence denotes Viral replication is inhibited in the presence of increasing concentration of GA, as determined by decreasing viral DNA copies.
T163 1031-1144 Sentence denotes DNA was extracted from 4 pooled wells of HFF at 7 dpi, then analyzed by qPCR targeting the viral polymerase gene.
T164 1145-1315 Sentence denotes DNA copies were reduced in a dose dependent manner by the addition of GA to culture medium as determined by ANOVA analysis with pairwise post-hoc t-test analysis in SPSS.
T165 1316-1414 Sentence denotes Data are presented as mean percent of control. (*5 µM p = 0.014, 8 µM p = 0.003, 10 µM p = 0.002).
T166 1415-1606 Sentence denotes 6 well cultures of HEp-2 (B) or 293T (C) were infected with HSV-1 strain F at an MOI of 1 for 2 hours, then washed with 199V and supplemented with 10 µM GA (Lanes 1–4) or vehicle (Lanes 5–8).
T167 1607-1817 Sentence denotes Mock (M), 5, 11 and 24 hours after inoculation, cells were collected and a fraction of the total cell lysate was subject to Western Blot analysis using antibodies directed against ICP8, ICP27, β-Actin and US11.
T168 1818-1880 Sentence denotes Normalized ratios of protein expression are in the bar graphs.
T169 1881-1965 Sentence denotes Viral protein synthesis was determined in HSV-infected cells following GA treatment.
T170 1966-2176 Sentence denotes Untreated HEp-2 and 293T cells were infected with HSV-1 F’ at an MOI of 1 for 2 hours, allowing the virions to internalize into the cells, then washed with 199V medium and supplemented with 10 µM GA or vehicle.
T171 2177-2257 Sentence denotes The viral replication was evaluated by Western Blot detection of HSV-1 proteins.
T172 2258-2464 Sentence denotes Although the infection of HEp-2 and 293T cells had been initiated, the addition of 10 µM GA to the infected HEp-2 and 293T cells inhibited the virus replication from the point of exposure to GA (Fig. 7B,C).
T173 2465-2543 Sentence denotes The observed effect could be the result of inhibition of secondary infections.
T174 2544-2747 Sentence denotes However, the early down regulation of HSV proteins, occurring prior to a complete viral replication cycle, suggests that there could be a secondary inhibitory mechanism targeting viral protein synthesis.