| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T254 |
0-640 |
Sentence |
denotes |
To build a comprehensive list of human PPIs, we assembled data from a total of 18 bioinformatics and systems biology databases with five types of experimental evidence: (i) binary PPIs tested by high-throughput yeast-two-hybrid (Y2H) systems; (ii) binary, physical PPIs from protein 3D structures; (iii) kinase-substrate interactions by literature-derived low-throughput or high-throughput experiments; (iv) signaling network by literature-derived low-throughput experiments; and (v) literature-curated PPIs identified by affinity purification followed by mass spectrometry (AP-MS), Y2H, or by literature-derived low-throughput experiments. |
| T255 |
641-757 |
Sentence |
denotes |
All inferred data, including evolutionary analysis, gene expression data, and metabolic associations, were excluded. |
| T256 |
758-915 |
Sentence |
denotes |
The genes were mapped to their Entrez ID based on the NCBI database92 as well as their official gene symbols based on GeneCards (https://www.genecards.org/). |
| T257 |
916-1153 |
Sentence |
denotes |
In total, the resulting human protein–protein interactome used in this study includes 351,444 unique PPIs (edges or links) connecting 17,706 proteins (nodes), representing a 50% increase in the number of the PPIs we have used previously. |
| T258 |
1154-1275 |
Sentence |
denotes |
Detailed descriptions for building the human protein–protein interactome are provided in our previous studies13,23,28,93. |
