| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T63 |
0-544 |
Sentence |
denotes |
Three additional one-step real-time PCR systems were compared with the initially used QuantiTect Virus +Rox Vial kit (QIAGEN) (Table): (i) OneStep RT-PCR kit (QIAGEN; data not shown in the Table because only a limited number of samples were tested in only one run), (ii) LightCycler Multiplex RNA Virus Master (Roche, Mannheim, Germany; data not shown because only a limited number of samples were tested in only one run) and (iii) SuperScript III One-Step RT-PCR system with Platinum TaqDNA Polymerase (Invitrogen, Darmstadt, Germany) (Table). |
| T64 |
545-707 |
Sentence |
denotes |
Each assay protocol and thermoprofile was adjusted to the recommendations of the manufacturer, but primer and probe concentrations were the same as published [5]. |
| T65 |
708-785 |
Sentence |
denotes |
They all showed comparable results in reduction of unspecific E gene signals. |
| T66 |
786-1147 |
Sentence |
denotes |
Using SuperScript III, the unspecific signals in the E gene screening assay were significantly reduced to 5% (13/257) in tested patient samples and negative extraction and non-template controls (Table), whereas Ct values for positive controls (Wuhan coronavirus 2019 E gene and SARS-CoV Frankfurt 1 genomic RNA [3]) were in the same range in QuantiTect (median: |
| T67 |
1148-1160 |
Sentence |
denotes |
29.1; range: |
| T68 |
1161-1207 |
Sentence |
denotes |
26.8–32.4; n = 85) and Superscript (median Ct: |
| T69 |
1208-1220 |
Sentence |
denotes |
28.1; range: |
| T70 |
1221-1247 |
Sentence |
denotes |
26.4–31.0; n = 30) setups. |
