| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T42 |
0-189 |
Sentence |
denotes |
Table Comparison of two different one-step real-time RT-PCR systems with SARS-CoV-2 assays from Corman et al. [5] and a commercial test kit with kit-specific assays, Bavaria, February 2020 |
| T43 |
190-399 |
Sentence |
denotes |
Real-time RT-PCR system PCR efficiency (%)a, linearity (R2) Limit of detection (copies/reaction) Unspecific signals count in E gene assay in totalb Unspecific signals in E gene assay (%)b Run time (hours) |
| T44 |
400-504 |
Sentence |
denotes |
QuantiTect Virus +Rox Vial kit (QIAGEN) ND ND 451/743(75/126 NC, 376/617 patient samples) 60.7 1:50 |
| T45 |
505-656 |
Sentence |
denotes |
SuperScript III One-step RT-PCR System with Platinum TaqDNA Polymerase (Invitrogen) 95 / 0,99c 50c 13/257(2/38 NC,11/219 patient samples) 5.1 1:28 |
| T46 |
657-766 |
Sentence |
denotes |
RealStar SARS-CoV-2 RT-PCR kit 1.0 (Altona) 125 / 0,97d 10d 0/111(0/38 NC, 0/73 patients samples) 0 2:15 |
| T47 |
767-816 |
Sentence |
denotes |
NC: negative control samples; ND: not determined. |
| T48 |
817-838 |
Sentence |
denotes |
a E = 10−1/slope − 1. |
| T49 |
839-1059 |
Sentence |
denotes |
b Indicated counts and percentage values of unspecific background signals in the SARS-CoV E gene assay are based on the total number of tested patient samples as well as the negative extraction and non-template controls. |
| T50 |
1060-1191 |
Sentence |
denotes |
c Only for RdRp gene assays, tested with four replicates of SARS-CoV Frankfurt 1 RNA [6]; 10-fold serial dilutions were determined. |
| T51 |
1192-1233 |
Sentence |
denotes |
For the E gene, the assay was not linear. |
| T52 |
1234-1415 |
Sentence |
denotes |
d Only for the E gene, tested with two replicates of synthetic Wuhan coronavirus 2019 E gene control and SARS-CoV Frankfurt 1 RNA each [6]; 10-fold serial dilutions were determined. |
