| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T16 |
0-165 |
Sentence |
denotes |
We initially used the protocol based on the E gene and RdRp gene developed by the German Consiliary Laboratory for Coronaviruses hosted at the Charité in Berlin [5]. |
| T17 |
166-363 |
Sentence |
denotes |
Real-time RT-PCR was initially performed with the QuantiTect Virus +Rox Vial kit (QIAGEN, Hilden, Germany) on the Bio-Rad CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Feldkirchen, Germany). |
| T18 |
364-486 |
Sentence |
denotes |
The kit was chosen for its frequent and successful use in our laboratory with other assays and its immediate availability. |
| T19 |
487-579 |
Sentence |
denotes |
Primers and probes were used as described [5] and provided by TIB Molbiol (Berlin, Germany). |
| T20 |
580-806 |
Sentence |
denotes |
Control material was ordered from the European Virus Archive (EVAg) and consisted of synthetic Wuhan coronavirus 2019 E gene control (reference number 026N-03866) and SARS-CoV Frankfurt 1 RNA (reference number 004N-02005) [6]. |
| T21 |
807-945 |
Sentence |
denotes |
In addition, the control of LightMix Modular Wuhan CoV RdRP-gene (TibMolbiol, Berlin, Germany) was used for the SARS-CoV-2 specific assay. |
| T22 |
946-1047 |
Sentence |
denotes |
Respiratory samples (nasopharyngeal swabs or sputum) were obtained from patients and contact persons. |
| T23 |
1048-1116 |
Sentence |
denotes |
Sputum samples were diluted in 2 mL phosphate buffered saline (PBS). |
| T24 |
1117-1236 |
Sentence |
denotes |
RNA was extracted using the QIAamp Bio Robot kit (QIAGEN) on a Hamilton Microlab Star (Hamilton, Bonaduz, Switzerland). |
