| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T102 |
0-268 |
Sentence |
denotes |
Contamination as a reason for unspecific signals was ruled out, as stringent prevention measures were taken, e.g. strict separation of working areas: oligonucleotides and PCR mastermix reagents were handled in one room under a PCR hood with specified laboratory coats. |
| T103 |
269-335 |
Sentence |
denotes |
Sample preparation and RNA extraction took place in a second room. |
| T104 |
336-390 |
Sentence |
denotes |
Sample RNA was added in a third room under a PCR hood. |
| T105 |
391-452 |
Sentence |
denotes |
The synthetic E gene control was added last to the mastermix. |
| T106 |
453-509 |
Sentence |
denotes |
All reagents were aliquoted and aliquots used once only. |
| T107 |
510-792 |
Sentence |
denotes |
Contaminations from synthetic E gene present in primer batches upon delivery can be ruled out as well, although only one batch of E gene primers and probes was used with the QuantiTect and Superscript III setup, as only a certain proportion of samples showed the unspecific signals. |
| T108 |
793-959 |
Sentence |
denotes |
Furthermore, the unspecific signals were significantly reduced in the Superscript III setup, which showed that its sensitivity was comparable to the QuantiTect setup. |
| T109 |
960-1208 |
Sentence |
denotes |
In addition, the initially used E gene primers and probe were separately used as templates with the RealStar kit and no amplification was observed, whereas the corresponding artificial E gene template delivered a clear S-shaped curve with this kit. |
