| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T39 |
0-118 |
Sentence |
denotes |
Limit of detection (LoD), inter-run variability and cross-reactivity with other respiratory pathogens were determined. |
| T40 |
119-363 |
Sentence |
denotes |
In-vitro transcribed RNA (IVT RNA) of the E gene of SARS-CoV-2 and purified RNA of SARS-CoV (strain Frankfurt-1) were used as positive controls (obtained via the European virus archive global (EVAg), https://www.european-virus-archive.com) [7]. |
| T41 |
364-682 |
Sentence |
denotes |
Limit of detection of the SARS-CoV-2 UCT was determined by analysing each of eight replicates of a dilution series containing IVT RNA diluted in E-swab medium (Copan, Brescia, Italy; modified liquid Amies medium) and Roche cobas PCR medium (1:1) at 10,000, 1,000, 500, 250 and 125 copies/mL and eight negative samples. |
| T42 |
683-793 |
Sentence |
denotes |
The LoD was 689.3 copies/mL with 275.72 copies per reaction at 95% detection probability (Figure 1, Figure 2). |
| T43 |
794-1007 |
Sentence |
denotes |
For estimation of inter/intra-run variability, we analysed each of two concentrations (ca 5 × and 10 × LoD spiked IVT SARS-CoV-2 RNA) in five replicates and a negative sample with five replicates in two runs each. |
| T44 |
1008-1106 |
Sentence |
denotes |
Minimal deviation was observed with ± 0.5 cycle threshold (Ct) at 10 × LoD and 0.75 Ct at 5 × LoD. |
| T45 |
1107-1142 |
Sentence |
denotes |
No false positive results occurred. |
