LitCovid-sentences  

PMC:7068162 / 4434-8118 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T38 0-54 Sentence denotes SARS-CoV-2 Utility Channel test performance evaluation
T39 55-173 Sentence denotes Limit of detection (LoD), inter-run variability and cross-reactivity with other respiratory pathogens were determined.
T40 174-418 Sentence denotes In-vitro transcribed RNA (IVT RNA) of the E gene of SARS-CoV-2 and purified RNA of SARS-CoV (strain Frankfurt-1) were used as positive controls (obtained via the European virus archive global (EVAg), https://www.european-virus-archive.com) [7].
T41 419-737 Sentence denotes Limit of detection of the SARS-CoV-2 UCT was determined by analysing each of eight replicates of a dilution series containing IVT RNA diluted in E-swab medium (Copan, Brescia, Italy; modified liquid Amies medium) and Roche cobas PCR medium (1:1) at 10,000, 1,000, 500, 250 and 125 copies/mL and eight negative samples.
T42 738-848 Sentence denotes The LoD was 689.3 copies/mL with 275.72 copies per reaction at 95% detection probability (Figure 1, Figure 2).
T43 849-1062 Sentence denotes For estimation of inter/intra-run variability, we analysed each of two concentrations (ca 5 × and 10 × LoD spiked IVT SARS-CoV-2 RNA) in five replicates and a negative sample with five replicates in two runs each.
T44 1063-1161 Sentence denotes Minimal deviation was observed with ± 0.5 cycle threshold (Ct) at 10 × LoD and 0.75 Ct at 5 × LoD.
T45 1162-1197 Sentence denotes No false positive results occurred.
T46 1198-1294 Sentence denotes Figure 1 Example for amplification curves of the SARS-CoV-2-UCT SARS-CoV-2 Utility Channel test
T47 1295-1329 Sentence denotes IVT RNA: in-vitro transcribed RNA.
T48 1330-1477 Sentence denotes Dark blue curves: specific SARS-CoV-2 signals of IVT-RNA at 100,000 copies/mL (1), 10,000 copies/ml (2), 1,000 copies/ml (3) and 100 copies/ml (4).
T49 1478-1533 Sentence denotes Light blue curves: signals of the internal control (5).
T50 1534-1652 Sentence denotes Figure 2 Determination of limit of detection of the SARS-CoV-2 Utility Channel test based on in-vitro transcribed RNA
T51 1653-1687 Sentence denotes IVT RNA: in-vitro transcribed RNA.
T52 1688-1772 Sentence denotes Probit analysis revealed a limit of detection of 689.3 copies/mL at 95% probability.
T53 1773-1836 Sentence denotes Blue line: probit curve (dose-response rule); red dotted lines:
T54 1837-1862 Sentence denotes 95% confidence intervals.
T55 1863-1888 Sentence denotes IVT-RNA obtained by EVAg.
T56 1889-2189 Sentence denotes Potential interference of the SARS-CoV-2 UCT with other respiratory pathogens (including other human CoV strains) was evaluated by analysing 88 previously determined clinical samples and an external quality control assessment panel (INSTAND, Düsseldorf, Germany) containing lysates of infected cells.
T57 2190-2373 Sentence denotes None of these organisms were detected by the SARS-CoV-2 UCT assay (Table 2), confirming high specificity of the assay for viruses within the Betacoronavirus subgenus Sarbecovirus [4].
T58 2374-2553 Sentence denotes Table 2 Potential cross-reactivity SARS-CoV-2 Utility Channel test with other respiratory pathogens, evaluated with a panel of organisms typically found in respiratory infections
T59 2554-2604 Sentence denotes Clinical samples with known viruses Number tested
T60 2605-2631 Sentence denotes Coronavirus (not typed) 5
T61 2632-2645 Sentence denotes hCoV HKU-1 2
T62 2646-2658 Sentence denotes hCoV NL63 1
T63 2659-2672 Sentence denotes Adenovirus 2
T64 2673-2685 Sentence denotes Bocavirus 7
T65 2686-2710 Sentence denotes Human metapneumovirus 6
T66 2711-2725 Sentence denotes Influenza A 7
T67 2726-2746 Sentence denotes Influenza A(H1N1) 6
T68 2747-2761 Sentence denotes Influenza B 3
T69 2762-2786 Sentence denotes Parainfluenza 1 virus 3
T70 2787-2811 Sentence denotes Parainfluenza 2 virus 1
T71 2812-2836 Sentence denotes Parainfluenza 3 virus 8
T72 2837-2861 Sentence denotes Parainfluenza 4 virus 3
T73 2862-2899 Sentence denotes Respiratory syncytial virus (A/B) 10
T74 2900-2920 Sentence denotes Rhino/enterovirus 8
T75 2921-2948 Sentence denotes Chlamydophila pneumoniae 4
T76 2949-2973 Sentence denotes Mycoplasma pneumoniae 4
T77 2974-2999 Sentence denotes Legionella pneumophila 3
T78 3000-3023 Sentence denotes Bordetella pertussis 4
T79 3024-3051 Sentence denotes Bordetella parapertussis 1
T80 3052-3089 Sentence denotes Total number of clinical samples 88
T81 3090-3178 Sentence denotes External quality control assessment panel with known viruses (lysates of infected cells)
T82 3179-3191 Sentence denotes hCoV 229E 1
T83 3192-3204 Sentence denotes hCoV NL63 1
T84 3205-3217 Sentence denotes hCoV OC43 3
T85 3218-3229 Sentence denotes MERS-CoV 4
T86 3230-3251 Sentence denotes Coxsackievirus A21 1
T87 3252-3272 Sentence denotes Coxsackievirus B3 1
T88 3273-3290 Sentence denotes Enterovirus 68 1
T89 3291-3304 Sentence denotes Rhinovirus 3
T90 3305-3329 Sentence denotes Human metapneumovirus 3
T91 3330-3347 Sentence denotes Virus-negative 4
T92 3348-3409 Sentence denotes Total number of quality control assessment panel samples 22
T93 3410-3439 Sentence denotes Total number of samples 110
T94 3440-3464 Sentence denotes hCoV: human coronavirus;
T95 3465-3526 Sentence denotes Clinical samples had been pre-analysed by routine diagnostic.
T96 3527-3684 Sentence denotes External quality control assessment panel (INSTAND) samples contained inactivated lysates of virus infected cells including four virus-negative cell lysates.