| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T15 |
0-144 |
Sentence |
denotes |
A custom-made primer/probe set based on a previously published assay targeting the E gene [4] was optimised for the use on the automated system. |
| T16 |
145-235 |
Sentence |
denotes |
Primer and probes were ordered from IDT DNA Technologies (Coralville, United States (US)). |
| T17 |
236-350 |
Sentence |
denotes |
Both primers were modified with 2’-O-methyl bases in their penultimate base to prevent formation of primer dimers. |
| T18 |
351-438 |
Sentence |
denotes |
The ZEN double-quenched probe (IDT) was used in order to lower background fluorescence. |
| T19 |
439-773 |
Sentence |
denotes |
The master mix (Mmx) cassette (for 96 tests) is prepared by combining 84 µL forward primer (400 nM, 5´-ACAGGTACGTTAATAGTTAATAGCmGT-3´), 84 µL reverse primer (400 nM, 5´-ATATTGCAGCAGTACGCACAmCA-3´) and 10.5 µL probe (50 nM, 5´-Fam-ACACTAGCC/ZEN/ATCCTTACTGCGCTTCG-Iowa Black FQ-3’)* together with 182 µL water and 5,640 µl Mmx2 mixture. |
| T20 |
774-837 |
Sentence |
denotes |
After mixing, the 6 mL are transferred to the reagent cassette. |
| T21 |
838-1020 |
Sentence |
denotes |
The Mmx cassette is delivered with a full-process control: it is preloaded with an internal control (IC) RNA together with primers and probe of the IC detection assay by default [6]. |
| T22 |
1021-1189 |
Sentence |
denotes |
Instrument settings in the cobas mni Utility Channel software (Roche, Los Gatos, US) and the temperature profile used for the RT-PCR reaction are summarised in Table 1. |
