| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T56 |
0-186 |
Sentence |
denotes |
In-house anti-SARSr-CoV IgG and IgM ELISA kits were developed using SARSr-CoV Rp3 NP as antigen, which shared above 90% amino acid identity to all SARSr-CoVs, as reported previously [5]. |
| T57 |
187-299 |
Sentence |
denotes |
For IgG test, MaxiSorp Nunc-immuno 96 well ELISA plates were coated (100 ng/well) overnight with recombinant NP. |
| T58 |
300-354 |
Sentence |
denotes |
Human sera were used at 1:20 dilution for 1 h at 37°C. |
| T59 |
355-481 |
Sentence |
denotes |
An anti-Human IgG-HRP conjugated monoclonal antibody (Kyab Biotech Co., Ltd, Wuhan, China) was used at a dilution of 1:40,000. |
| T60 |
482-520 |
Sentence |
denotes |
The OD value (450–630) was calculated. |
| T61 |
521-642 |
Sentence |
denotes |
For IgM test, MaxiSorp Nunc-immuno 96 wellELISA plates were coated (500 ng/well) overnight with anti-human IgM (µ chain). |
| T62 |
643-803 |
Sentence |
denotes |
Human sera were used at 1:100 dilution for 40 min at 37°C, followed by anti-Rp3 NP-HRP conjugated (Kyab Biotech Co., Ltd, Wuhan, China) at a dilution of 1:4000. |
| T63 |
804-842 |
Sentence |
denotes |
The OD value (450–630) was calculated. |
