Id |
Subject |
Object |
Predicate |
Lexical cue |
T86 |
0-2 |
Sentence |
denotes |
3. |
T87 |
3-47 |
Sentence |
denotes |
Full-length genome and phylogenetic analysis |
T88 |
48-187 |
Sentence |
denotes |
After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium. |
T89 |
188-288 |
Sentence |
denotes |
The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq. |
T90 |
289-368 |
Sentence |
denotes |
De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene. |
T91 |
369-640 |
Sentence |
denotes |
The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3). |
T92 |
641-836 |
Sentence |
denotes |
The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of > 99.5% with other isolated SARS-CoV-2 sequences. |
T93 |
837-985 |
Sentence |
denotes |
The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193). |