PMC:7045880 / 7868-8750 JSONTXT 5 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T74 0-2 Sentence denotes 1.
T75 3-34 Sentence denotes Virus isolation from Vero cells
T76 35-245 Sentence denotes Following inoculation of Vero cells with the nasopharyngeal and oropharyngeal samples, they were observed at 24-hour intervals, and the cytopathic effects were observed from 3 days after inoculation (Figure 1).
T77 246-358 Sentence denotes The inoculated cells were harvested on the 4th day when more than 80% of the cells exhibited cytopathic effects.
T78 359-462 Sentence denotes Virus replication was confirmed using real-time RT-PCR with RNA extracted from the cell culture medium.
T79 463-665 Sentence denotes The Ct values were 14.40 and 18.26 for the nasopharyngeal and oropharyngeal samples, respectively, which were lower than the cycle threshold (Ct) values of 20.85 and 21.85 in the pre-inoculated samples.
T80 666-882 Sentence denotes The number of virus copies in the samples before inoculation was 7.6 × 108 and 3.9 × 108 copy/mL, respectively, and increased by 10–70-fold to 5.4 × 1010 and 4.2 × 109, respectively, in the cell culture supernatants.