Id |
Subject |
Object |
Predicate |
Lexical cue |
T74 |
0-2 |
Sentence |
denotes |
1. |
T75 |
3-34 |
Sentence |
denotes |
Virus isolation from Vero cells |
T76 |
35-245 |
Sentence |
denotes |
Following inoculation of Vero cells with the nasopharyngeal and oropharyngeal samples, they were observed at 24-hour intervals, and the cytopathic effects were observed from 3 days after inoculation (Figure 1). |
T77 |
246-358 |
Sentence |
denotes |
The inoculated cells were harvested on the 4th day when more than 80% of the cells exhibited cytopathic effects. |
T78 |
359-462 |
Sentence |
denotes |
Virus replication was confirmed using real-time RT-PCR with RNA extracted from the cell culture medium. |
T79 |
463-665 |
Sentence |
denotes |
The Ct values were 14.40 and 18.26 for the nasopharyngeal and oropharyngeal samples, respectively, which were lower than the cycle threshold (Ct) values of 20.85 and 21.85 in the pre-inoculated samples. |
T80 |
666-882 |
Sentence |
denotes |
The number of virus copies in the samples before inoculation was 7.6 × 108 and 3.9 × 108 copy/mL, respectively, and increased by 10–70-fold to 5.4 × 1010 and 4.2 × 109, respectively, in the cell culture supernatants. |