LitCovid-sentences  

PMC:7045880 / 7859-10111 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T73 0-7 Sentence denotes Results
T74 9-11 Sentence denotes 1.
T75 12-43 Sentence denotes Virus isolation from Vero cells
T76 44-254 Sentence denotes Following inoculation of Vero cells with the nasopharyngeal and oropharyngeal samples, they were observed at 24-hour intervals, and the cytopathic effects were observed from 3 days after inoculation (Figure 1).
T77 255-367 Sentence denotes The inoculated cells were harvested on the 4th day when more than 80% of the cells exhibited cytopathic effects.
T78 368-471 Sentence denotes Virus replication was confirmed using real-time RT-PCR with RNA extracted from the cell culture medium.
T79 472-674 Sentence denotes The Ct values were 14.40 and 18.26 for the nasopharyngeal and oropharyngeal samples, respectively, which were lower than the cycle threshold (Ct) values of 20.85 and 21.85 in the pre-inoculated samples.
T80 675-891 Sentence denotes The number of virus copies in the samples before inoculation was 7.6 × 108 and 3.9 × 108 copy/mL, respectively, and increased by 10–70-fold to 5.4 × 1010 and 4.2 × 109, respectively, in the cell culture supernatants.
T81 893-895 Sentence denotes 2.
T82 896-958 Sentence denotes Analysis of the structure of the virus by electron micrographs
T83 959-1082 Sentence denotes The structure of the virus in the cytoplasm of 3-day post-inoculation cells was examined by electron microscopy (Figure 2).
T84 1083-1128 Sentence denotes Coronavirus-specific morphology was observed.
T85 1129-1265 Sentence denotes Virus particle size ranged from 70–90 nm and the virus was observed in a wide range of intracellular organelles, especially in vesicles.
T86 1267-1269 Sentence denotes 3.
T87 1270-1314 Sentence denotes Full-length genome and phylogenetic analysis
T88 1315-1454 Sentence denotes After inoculating cells with the nasopharyngeal and oropharyngeal samples, RNA was extracted from the virus-replicated cell culture medium.
T89 1455-1555 Sentence denotes The RNA was amplified with primers for full-length gene analysis, and NGS was performed using Miseq.
T90 1556-1635 Sentence denotes De novo assembly of the NGS sequence secured 28,818 bp of the full-length gene.
T91 1636-1907 Sentence denotes The acquired gene was compared with 57 human and animal coronaviruses, including Wuhan/IVDC-HB-01/2019(GISAID accession ID: EPI_ISL_402119~121), which was first reported in Wuhan, 54 reported full-length SARS-CoV-2 genes, Bat-SARS-like CoV, and human SARS-CoV (Figure 3).
T92 1908-2103 Sentence denotes The analysis showed that the sequence was included in the same cluster as the previously reported SARS-CoV-2 sequence and showed high homology of > 99.5% with other isolated SARS-CoV-2 sequences.
T93 2104-2252 Sentence denotes The virus was named BetaCoV/Korea/KCDC03/2020, and its full-length gene sequence was registered in WHO GISAID (GISAID accession ID: EPI_ISL_407193).