| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T64 |
0-2 |
Sentence |
denotes |
6. |
| T65 |
3-35 |
Sentence |
denotes |
Transmission electron microscopy |
| T66 |
36-263 |
Sentence |
denotes |
For transmission electron microscopy, the inoculated cells were prefixed by incubating in 2% paraformaldehyde and 2.5% glutaraldehyde in 0.1 M phosphate buffer (pH 7.4) to prevent the autolysis of the cells infected with virus. |
| T67 |
264-451 |
Sentence |
denotes |
To minimize the chemical reaction between pre- and post-fixation, the slides were washed 3 times using the same buffer as in the fixative solution and post-fixed with 1% osmium tetroxide. |
| T68 |
452-553 |
Sentence |
denotes |
After washing 3 times with deionized water, en bloc staining was performed using 0.5% uranyl acetate. |
| T69 |
554-718 |
Sentence |
denotes |
Thereafter, 30%, 50%, 70%, 80%, 90%, and 100% ethanol were used sequentially in ascending concentration for dehydration, which was substituted with propylene oxide. |
| T70 |
719-812 |
Sentence |
denotes |
The slides were then embedded in Epon812 plastic resin, and polymerized at 70°C for 48 hours. |
| T71 |
813-977 |
Sentence |
denotes |
The prepared plastic block was cut to 70-nm thick sections using an ultramicrotome and mounted on a 100-mesh nickel grid, and electrostained with 5% uranyl acetate. |
| T72 |
978-1122 |
Sentence |
denotes |
The sections were observed with a transmission electron microscope (Libra120, Carl Zeiss, Germany) at an acceleration voltage of 120 kV [13–15]. |
