| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T55 |
0-133 |
Sentence |
denotes |
Using reverse transcriptase, cDNA was synthesized from RNA extracted from the cultured cell medium in which the virus was replicated. |
| T56 |
134-339 |
Sentence |
denotes |
A next generation sequencing (NGS) library was constructed after amplifying the full-length genes of the isolates using the synthesized cDNA and primers designed based on published SARS-CoV-2 DNA sequence. |
| T57 |
340-405 |
Sentence |
denotes |
The prepared library was purified and analyzed with Miseq 150 PE. |
| T58 |
406-507 |
Sentence |
denotes |
De novo assembly was performed on the sequenced product using Megahit to secure a full-length genome. |
