Id |
Subject |
Object |
Predicate |
Lexical cue |
T46 |
0-2 |
Sentence |
denotes |
3. |
T47 |
3-18 |
Sentence |
denotes |
Virus isolation |
T48 |
19-120 |
Sentence |
denotes |
The virus was isolated from nasopharyngeal and oropharyngeal samples from putative COVID-19 patients. |
T49 |
121-366 |
Sentence |
denotes |
Oropharyngeal samples were diluted with viral transfer medium containing nasopharyngeal swabs and antibiotics (Nystadin, penicillin-streptomycin 1:1 dilution) at 1:4 ratio and incubated for 1 hour at 4°C, before being inoculated onto Vero cells. |
T50 |
367-535 |
Sentence |
denotes |
Inoculated Vero cells were cultured at 37°C, 5% CO2 in 1× Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 2% fetal bovine serum and penicillin-streptomycin. |
T51 |
536-651 |
Sentence |
denotes |
Virus replication and isolation were confirmed through cytopathic effects, gene detection, and electron microscopy. |
T52 |
652-823 |
Sentence |
denotes |
Viral culture of SARS-CoV-2 was conducted in a biosafety Level-3 facility according to laboratory biosafety guidelines of Korea Centers for Disease Control and Prevention. |