Id |
Subject |
Object |
Predicate |
Lexical cue |
T46 |
0-140 |
Sentence |
denotes |
Total RNA extracted from BALF samples (collected on 2nd January 2020) were subject to metagenomic next-generation sequencing (mNGS) testing. |
T47 |
141-480 |
Sentence |
denotes |
The concentration of RNA samples were low (<0.5 ng/ul) based on measurement by Qubit RNA HS Assay Kit (Thermo Fisher Scientific), and therefore the library preparation was performed with Trio RNA-Seq kit (NuGEN Technologies, USA) which targeted low concentration RNA samples and contained AnyDeplete probe that removes human ribosomal RNA. |
T48 |
481-580 |
Sentence |
denotes |
The resulting libraries were subject to 150 bp pair-end sequencing with an Illumina Miseq platform. |
T49 |
581-636 |
Sentence |
denotes |
The sequencing results were obtained in less than 24 h. |