PMC:7033720 / 11963-13139 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T113 0-171 Sentence denotes On 7th January 2020, the sequencing results were obtained in less than 24 h, with 7,369,020 and 4,522,558 reads generated for the samples of patient 1 and 2, respectively.
T114 172-328 Sentence denotes To identify potential pathogens from the mNGS sequencing results, a pathogen discovery pipeline based on individual reads was carried out on sequenced data.
T115 329-507 Sentence denotes Aside from those belonged to PhiX genome (in-library control), a majority of the viral reads (99.9% and 99.7% respectively for sample 1 and 2) were associated with coronaviruses.
T116 508-650 Sentence denotes The raw sequence data minus human genomic information was uploaded to Sequence Read Archive (SRA) database (Bioproject accession PRJNA601736).
T117 651-907 Sentence denotes On the other hand, bacterial pathogen identification was carried out by using the Metaphlan2 program, which revealed Capnocytophaga sp and Veillonella sp in sample 2 and none in sample 1, and both bacteria identified were not known for their pathogenicity.
T118 908-999 Sentence denotes Collectively, coronavirus is likely to be the main microbial pathogen within these samples.
T119 1000-1102 Sentence denotes The reads were assembled de novo using Megahit to form a ∼30 kb contigs with sequence homology to CoV.
T120 1103-1176 Sentence denotes After confirmation with read mapping, the final CoV genome was 29,881 nt.