Id |
Subject |
Object |
Predicate |
Lexical cue |
T113 |
0-171 |
Sentence |
denotes |
On 7th January 2020, the sequencing results were obtained in less than 24 h, with 7,369,020 and 4,522,558 reads generated for the samples of patient 1 and 2, respectively. |
T114 |
172-328 |
Sentence |
denotes |
To identify potential pathogens from the mNGS sequencing results, a pathogen discovery pipeline based on individual reads was carried out on sequenced data. |
T115 |
329-507 |
Sentence |
denotes |
Aside from those belonged to PhiX genome (in-library control), a majority of the viral reads (99.9% and 99.7% respectively for sample 1 and 2) were associated with coronaviruses. |
T116 |
508-650 |
Sentence |
denotes |
The raw sequence data minus human genomic information was uploaded to Sequence Read Archive (SRA) database (Bioproject accession PRJNA601736). |
T117 |
651-907 |
Sentence |
denotes |
On the other hand, bacterial pathogen identification was carried out by using the Metaphlan2 program, which revealed Capnocytophaga sp and Veillonella sp in sample 2 and none in sample 1, and both bacteria identified were not known for their pathogenicity. |
T118 |
908-999 |
Sentence |
denotes |
Collectively, coronavirus is likely to be the main microbial pathogen within these samples. |
T119 |
1000-1102 |
Sentence |
denotes |
The reads were assembled de novo using Megahit to form a ∼30 kb contigs with sequence homology to CoV. |
T120 |
1103-1176 |
Sentence |
denotes |
After confirmation with read mapping, the final CoV genome was 29,881 nt. |