LitCovid-sentences  

PMC:7019868 / 7390-9327 JSONTXT 7 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function IAV-Glycan

Id Subject Object Predicate Lexical cue
T52 0-4 Sentence denotes 2.3.
T53 5-110 Sentence denotes Generation and Recovery of Recombinant iPEDVPT-P5, iPEDVPT-P96, iPEDVPT-P5-96S and iPEDVPT-P96-5S Viruses
T54 111-187 Sentence denotes The strategy used to recover iPEDVPT-P96 has been described previously [19].
T55 188-297 Sentence denotes The approach to constructing a cDNA clone of iPEDVPT-P5 was technically identical to that of the iPEDVPT-P96.
T56 298-565 Sentence denotes However, we split the plasmid B into two fragments because the sequence remained toxic to the One Shot™ TOP10 Chemically Competent E. coli cells (Invitrogen, Carlsbad, USA) despite propagation in LB broth supplemented with 10% SOC medium and being incubated at 30 °C.
T57 566-764 Sentence denotes To generate chimeric viruses carrying heterologous spike (S) genes, namely the iPEDVPT-P5-96S and iPEDVPT-P96-5S, cDNA clones of iPEDVPT-P5 and iPEDVPT-P96, respectively, were used as the backbones.
T58 765-891 Sentence denotes The sequences covering the complete S gene of each virus were exchanged without disruption to the remaining genomic structure.
T59 892-992 Sentence denotes Sequence differences in the S genes of iPEDVPT-P5 and iPEDVPT-P96 viruses are summarized in Table 1.
T60 993-1185 Sentence denotes Each plasmid was digested with corresponding type-IIS restriction enzymes as designated in Figure S1, gel-purified, assembled and phenol-chloroform extracted to generate the full-length cDNAs.
T61 1186-1504 Sentence denotes The cDNAs were then in vitro transcribed to the full-length RNA transcripts using a mMessage mMachine T7 transcription kit (Ambion, Austin, CA, USA) and immediately electroporated into 800 μL of 107 cells/mL Vero cells in RNase-free phosphate buffered saline (PBS) along with 5 μg of PEDV nucleocapsid (N) transcripts.
T62 1505-1697 Sentence denotes After electroporation, the cells were allowed to recover in growth medium for approximately 16 h and then maintained in PI-medium until cytopathic effects involved over 90% of cell monolayers.
T63 1698-1832 Sentence denotes The whole flasks were subjected to one freeze-and-thaw cycle and the rescued viruses were passaged once to generate viral stocks (P1).
T64 1833-1937 Sentence denotes The viral stocks were titrated on Vero cells in 96-well plates to determine the viral titer (see below).