LitCovid-sentences  

PMC:7019868 / 25849-29029 JSONTXT 7 Projects

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Id Subject Object Predicate Lexical cue
T178 0-124 Sentence denotes In the present study, the first pair of cDNA clones of a virulent G2b PEDV and its derived attenuated strain were generated.
T179 125-364 Sentence denotes Since these two viruses were closely related to each other, this established platform would allow for easier manipulate in studying the effect of single nucleotide polymorphism for identifying potential virulent/attenuating determinate(s).
T180 365-498 Sentence denotes In the present study, we demonstrated that reversion of virulence or attenuation occurred after the S gene was reciprocally replaced.
T181 499-768 Sentence denotes However, this finding is in disagreement with previously published results by Wang et al. [17], indicating that there was an absence of virulence reversion after S gene substitution between the highly virulent G2b strain, BJ2011C, and the avirulent G1a strain, CHM2013.
T182 769-868 Sentence denotes There could be a few reasons for the discrepancy between our observations that of previous studies.
T183 869-917 Sentence denotes First, the autonomy of the viruses is different.
T184 918-1167 Sentence denotes Viral attenuation in serial cell-culture or animal passage is a progressive process that involves a series of gene mutations and the subsequent alternated cooperative interplay between gene products and mechanisms influencing cell-virus interaction.
T185 1168-1399 Sentence denotes Although the patterns of attenuating mutation are similar among G2b PEDV strains, the asynchronous mutations between different PEDV strains could lead to loss of the cooperative or complementary function(s) for other gene products.
T186 1400-1487 Sentence denotes In the previous study, the used viral strains belonged to different genogroups [17,28].
T187 1488-1587 Sentence denotes Efficient viral assembly requires proper signaling and interaction between each structural protein.
T188 1588-1884 Sentence denotes Since the BJ2011C virus belonged to genogroup 2b, we speculated that the reason a singular S gene exchange failed to reverse the virulence of the avirulent G1a CHM2013 strain might be due to, at least in part, the suboptimal cooperation between structural proteins in terms of PEDV morphogenesis.
T189 1885-1975 Sentence denotes Second, the degree of attenuation differed between the CHM2013 virus and PEDVPT-P96 virus.
T190 1976-2255 Sentence denotes Comparing to the avirulent CHM2013 virus that induced no detectable viral shedding in two-day-old piglets, iPEDVPT-P96 virus retained some levels of virulence as it caused viral shedding, observable clinical symptoms, and even mortality in the seven-day-old conventional piglets.
T191 2256-2486 Sentence denotes Besides the S gene [17,28], the abolishment of the function of non-structural proteins (NSPs) alone has been demonstrated to attenuate highly virulent PEDVs by disrupting the antagonistic ability of host interferons (IFN) [16,27].
T192 2487-2606 Sentence denotes That means, when the function of certain NSP was abated, PEDVs lose its virulence, regardless of the S gene they carry.
T193 2607-2821 Sentence denotes Therefore, variations of NSPs in the CHM2013 virus might have a critical effect on its virulence, and the effect might be stronger than that contributed by the S gene derived from the highly virulent BJ2011C virus.
T194 2822-3098 Sentence denotes Similarly, we also speculated that the IFN-suppressive function provided by NSP(s) might also contribute to virulent differences between the iPEDVPT-P5-96S and iPEDVPT-P96 since nine mutations were identified in NSPs in the iPEDVPT-P96 as compared with that of PEDVPT-P5 [18].
T195 3099-3180 Sentence denotes Regardless, further studies are needed to clarify the hypotheses mentioned above.