| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T167 |
0-158 |
Sentence |
denotes |
Porcine epidemic diarrhea (PED) to date remains a colossal burden to the global swine industries owing to the lack of successful vaccine in the field [12,13]. |
| T168 |
159-465 |
Sentence |
denotes |
Moreover, other emerging and re-emerging swine enteric coronaviruses, including porcine deltacoronavirus (PDCoV) [24], and swine acute diarrhea syndrome coronavirus (SADS-CoV) [25], further complicate the field condition by affecting diagnostic accuracy and increasing the risk of viral recombination [26]. |
| T169 |
466-600 |
Sentence |
denotes |
Hence, there is still a pressing need to develop a safe and effective vaccine, particularly the LAV, to mount this disastrous disease. |
| T170 |
601-758 |
Sentence |
denotes |
The reverse genetics system is a powerful and widely used tool to study viral pathogenesis and novel LAV design by active modification of genes of interests. |
| T171 |
759-942 |
Sentence |
denotes |
Previous studies using reverse genetics on other coronaviruses have identified many virulent/attenuating determinants that might be shared among different genus of coronaviruses [12]. |
| T172 |
943-1080 |
Sentence |
denotes |
Nevertheless, direct evidences of attenuation due to harboring those mutated determinant(s) in pigs are still limited in PEDV [16,23,27]. |
| T173 |
1081-1405 |
Sentence |
denotes |
In the present study, to investigate the role of the S gene in the attenuation process of the PEDVPT 52 strain, we generated four infectious cDNA clones of G2b PEDV, including the parental virulent iPEDVPT-P5, attenuated iPEDVPT-P96, as well as two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) with exchanged S gene. |
| T174 |
1406-1637 |
Sentence |
denotes |
We found that the iPEDVPT-P96 virus fully regained the virulence after the exchange of S gene derived from the highly virulent iPEDVPT-P5 virus, showing comparable patterns of viral shedding, diarrhea and histopathological changes. |
| T175 |
1638-1857 |
Sentence |
denotes |
Our data confirmed that the S gene is the primary attenuating determinant of the iPEDVPT-P96 virus and the genetic backbone other than the S gene involving the attenuation of the iPEDVPT-P96 might be of less importance. |
| T176 |
1858-2037 |
Sentence |
denotes |
However, the iPEDVPT-P5-96S still exhibited partial virulence resulting in severer villous attenuation and higher mortality rate compared to those caused by the iPEDVPT-P96 virus. |
| T177 |
2038-2114 |
Sentence |
denotes |
This result supports that the virulence of PEDV might be a multigenic event. |
