| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T3 |
0-181 |
Sentence |
denotes |
Porcine epidemic diarrhea virus (PEDV) has continuously caused severe economic losses to the global swine industries; however, no successful vaccine against PEDV has been developed. |
| T4 |
182-475 |
Sentence |
denotes |
In this study, we generated four autologous recombinant viruses, including the highly virulent iPEDVPT-P5, attenuated iPEDVPT-P96, and two chimeric viruses (iPEDVPT-P5-96S and iPEDVPT-P96-5S) with the reciprocally exchanged spike (S) gene, to study the role of the S gene in PEDV pathogenesis. |
| T5 |
476-616 |
Sentence |
denotes |
A deeper understanding of PEDV attenuation will aid in the rational design of a live attenuated vaccine (LAV) using reverse genetics system. |
| T6 |
617-872 |
Sentence |
denotes |
Our results showed that replacing the S gene from the highly virulent iPEDVPT-P5 led to complete restoration of virulence of the attenuated iPEDVPT-P96, with nearly identical viral shedding, diarrhea pattern, and mortality rate as the parental iPEDVPT-P5. |
| T7 |
873-1154 |
Sentence |
denotes |
In contrast, substitution of the S gene with that from the attenuated iPEDVPT-P96 resulted in partial attenuation of iPEDVPT-P5, exhibiting similar viral shedding and diarrhea patterns as the parental iPEDVPT-P96 with slightly severe histological lesions and higher mortality rate. |
| T8 |
1155-1284 |
Sentence |
denotes |
Collectively, our data confirmed that the attenuation of the PEDVPT-P96 virus is primarily attributed to mutations in the S gene. |
| T9 |
1285-1373 |
Sentence |
denotes |
However, mutation in S gene alone could not fully attenuate the virulence of iPEDVPT-P5. |
| T10 |
1374-1449 |
Sentence |
denotes |
Gene (s) other than S gene might also play a role in determining virulence. |
