| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T115 |
0-80 |
Sentence |
denotes |
Immunohistochemistry was performed to evaluate the distribution of PEDV antigen. |
| T116 |
81-328 |
Sentence |
denotes |
Briefly, formalin-fixed paraffin-embedded tissues were sectioned at 4 μm, deparaffined in xylene, rehydrated in serially diluted ethanol, and proceeded to epitope retrieval with the Trilogy antigen retrieval system (Cell Marque, Rocklin, CA, USA). |
| T117 |
329-637 |
Sentence |
denotes |
After being washed three times with Tris-buffered saline plus 0.1% Tween 20 (TBST), tissue slides were treated with 3% hydrogen peroxidase (KYB, New Taipei City, Taiwan) and 10% normal goat serum (Dako, Carpinteria, CA, USA) to block the endogenous peroxidase activity and non-specific signals, respectively. |
| T118 |
638-829 |
Sentence |
denotes |
For antigen detection, an in-house anti-PEDV N antibody, DE-1, at a dilution of 1:1000 in 10% normal goat serum was applied to the slides for 1 h at RT followed by three times wash with TBST. |
| T119 |
830-1128 |
Sentence |
denotes |
The first antibodies were then captured using the polyclonal anti-rabbit/mouse immunoglobulin, EnVision-DAB+ system (Agilent Technologies, Santa Clara, CA, USA) at RT for 1 h and color was developed afterward with 3, 3′-diaminobenzidine (DAB) chromogen (Agilent Technologies, Santa Clara, CA, USA). |
| T120 |
1129-1265 |
Sentence |
denotes |
The slides were counterstained with hematoxylin (MUTO, Tokyo, Japan), mounted in Entellan (Merck, Darmstadt, Germany) and cover slipped. |
| T121 |
1266-1356 |
Sentence |
denotes |
Positive signals were visualized under an inverted light microscope (Nikon, Tokyo, Japan). |
