LitCovid-sentences  

PMC:7019868 / 12956-17051 JSONTXT 11 Projects

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Id Subject Object Predicate Lexical cue
T93 0-4 Sentence denotes 2.5.
T94 5-22 Sentence denotes Animal Experiment
T95 23-339 Sentence denotes Thirty-seven, six-day-old, Large White × Duroc, crossbred, fecal PEDV and TGEV shedding-negative suckling piglets were purchased from a conventional pig farm devoid of G2b PEDV infection history based on the negative result of our long-term surveillance of serum antibody and colostrum against PEDV in this pig farm.
T96 340-602 Sentence denotes These piglets from different sows were fed with artificial milk and were randomly assigned to five groups, acclimated for one day, and then inoculated orally with indicative recombinant viruses at a dose of 2 mL of 0.5 × 102 TCID50/mL or PI-medium, respectively.
T97 603-654 Sentence denotes Clinical signs and weight gain were recorded daily.
T98 655-716 Sentence denotes Fecal consistency was monitored daily and scored visually as:
T99 717-795 Sentence denotes 0 = normal, 1 = loose, 2 = semi-fluid, and 3 = watery as previously described.
T100 796-900 Sentence denotes Calculation of average daily weight gain was only performed on piglets that were not humanly euthanized.
T101 901-948 Sentence denotes The formula used for calculation is as follows:
T102 949-981 Sentence denotes Weight gained/ surviving period.
T103 983-989 Sentence denotes 2.5.1.
T104 990-1033 Sentence denotes Quantification of PEDV Fecal Viral Shedding
T105 1034-1115 Sentence denotes Methods to quantify fecal PEDV viral shedding has been described previously [19].
T106 1116-1376 Sentence denotes Briefly, fecal samples collected from rectal swabs were resuspended in DPBS and then subjected to automated nucleic acid extraction using Cador Pathogen 96 QIAcube HT Kit with QIAcube (Qiagen Inc., Hilden, Germany) according to the manufacturer’s instructions.
T107 1377-1668 Sentence denotes Complementary DNA was synthesized via reverse transcription using QuantiNova™ Reverse Transcription kit (Qiagen Inc., Hilden, Germany) and proceeded to quantitative real-time PCR analysis using the primer-probe set published previously on a CFX96 Thermal Cycler (Bio-Rad, Hercules, CA, USA).
T108 1669-1806 Sentence denotes The thermal profile comprised an initial denaturation at 95 °C for 2 min and then 45 cycles of 95 °C for 15 s followed by 60 °C for 15 s.
T109 1807-2046 Sentence denotes The detection limit of the assay was determined by generating standard curves from serial 10-fold dilutions of known amounts of in vitro transcribed RNA followed by reverse transcription and real-time PCR quantification as described above.
T110 2047-2113 Sentence denotes The detection limit was calculated as 4.8 log10 RNA copies per mL.
T111 2115-2121 Sentence denotes 2.5.2.
T112 2122-2161 Sentence denotes Histopathology and Immunohistochemistry
T113 2162-2420 Sentence denotes At three days post-inoculation, three pigs from each virus-treated group and one pig from mock group were humanely euthanized by electrocution followed by exsanguination for histopathological and immunohistochemical assessments, as described previously [22].
T114 2421-2738 Sentence denotes Duodenum, jejunum, ileum, cecum, colon, rectum and mesenteric lymph nodes were collected, formalin-fixed, paraffin-embedded, sectioned at 4 μm, and stained routinely with hematoxylin and eosin (H&E) for morphometric analysis by assessing the ratio of villi height to crypt depth blindly by one veterinary pathologist.
T115 2739-2819 Sentence denotes Immunohistochemistry was performed to evaluate the distribution of PEDV antigen.
T116 2820-3067 Sentence denotes Briefly, formalin-fixed paraffin-embedded tissues were sectioned at 4 μm, deparaffined in xylene, rehydrated in serially diluted ethanol, and proceeded to epitope retrieval with the Trilogy antigen retrieval system (Cell Marque, Rocklin, CA, USA).
T117 3068-3376 Sentence denotes After being washed three times with Tris-buffered saline plus 0.1% Tween 20 (TBST), tissue slides were treated with 3% hydrogen peroxidase (KYB, New Taipei City, Taiwan) and 10% normal goat serum (Dako, Carpinteria, CA, USA) to block the endogenous peroxidase activity and non-specific signals, respectively.
T118 3377-3568 Sentence denotes For antigen detection, an in-house anti-PEDV N antibody, DE-1, at a dilution of 1:1000 in 10% normal goat serum was applied to the slides for 1 h at RT followed by three times wash with TBST.
T119 3569-3867 Sentence denotes The first antibodies were then captured using the polyclonal anti-rabbit/mouse immunoglobulin, EnVision-DAB+ system (Agilent Technologies, Santa Clara, CA, USA) at RT for 1 h and color was developed afterward with 3, 3′-diaminobenzidine (DAB) chromogen (Agilent Technologies, Santa Clara, CA, USA).
T120 3868-4004 Sentence denotes The slides were counterstained with hematoxylin (MUTO, Tokyo, Japan), mounted in Entellan (Merck, Darmstadt, Germany) and cover slipped.
T121 4005-4095 Sentence denotes Positive signals were visualized under an inverted light microscope (Nikon, Tokyo, Japan).