PMC:7019868 / 11306-12180 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE Lectin_function

Id Subject Object Predicate Lexical cue
T81 0-174 Sentence denotes Confluent monolayers of Vero cells were seeded onto six-well plates (5 × 105 cells/well) and infected with each virus at a MOI of 1 and 0.001 at 37 °C for 1 h in triplicates.
T82 175-285 Sentence denotes The cells were then washed twice with Dulbecco’s phosphate-buffered saline (DPBS) and maintained in PI medium.
T83 286-483 Sentence denotes The supernatants at indicated time points were collected and proceeded for viral quantification on Vero cells in 96-well plates using the standard 50% tissue-culture infectious dose (TCID50) assay.
T84 484-687 Sentence denotes In brief, Vero cells in 96-well plates were washed twice with DPBS and then incubated with a 10-fold serially diluted culture supernatant acquired from the aforementioned six-well plate at 37 °C for 1 h.
T85 688-789 Sentence denotes After absorption, the inoculum was removed and replaced with fresh PI-medium following one wash step.
T86 790-874 Sentence denotes The titers were determined at 72 h post-infection using the Reed–Muench method [21].