Id |
Subject |
Object |
Predicate |
Lexical cue |
T81 |
0-174 |
Sentence |
denotes |
Confluent monolayers of Vero cells were seeded onto six-well plates (5 × 105 cells/well) and infected with each virus at a MOI of 1 and 0.001 at 37 °C for 1 h in triplicates. |
T82 |
175-285 |
Sentence |
denotes |
The cells were then washed twice with Dulbecco’s phosphate-buffered saline (DPBS) and maintained in PI medium. |
T83 |
286-483 |
Sentence |
denotes |
The supernatants at indicated time points were collected and proceeded for viral quantification on Vero cells in 96-well plates using the standard 50% tissue-culture infectious dose (TCID50) assay. |
T84 |
484-687 |
Sentence |
denotes |
In brief, Vero cells in 96-well plates were washed twice with DPBS and then incubated with a 10-fold serially diluted culture supernatant acquired from the aforementioned six-well plate at 37 °C for 1 h. |
T85 |
688-789 |
Sentence |
denotes |
After absorption, the inoculum was removed and replaced with fresh PI-medium following one wash step. |
T86 |
790-874 |
Sentence |
denotes |
The titers were determined at 72 h post-infection using the Reed–Muench method [21]. |