| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T42 |
0-452 |
Sentence |
denotes |
A 25 μL reaction contained 5 μL of RNA, 12.5 μL of 2 × reaction buffer provided with the Superscript III one step RT-PCR system with Platinum Taq Polymerase (Invitrogen, Darmstadt, Germany; containing 0.4 mM of each deoxyribont triphosphates (dNTP) and 3.2 mM magnesium sulphate), 1 μL of reverse transcriptase/Taq mixture from the kit, 0.4 μL of a 50 mM magnesium sulphate solution (Invitrogen), and 1 μg of nonacetylated bovine serum albumin (Roche). |
| T43 |
453-538 |
Sentence |
denotes |
Primer and probe sequences, as well as optimised concentrations are shown in Table 1. |
| T44 |
539-623 |
Sentence |
denotes |
All oligonucleotides were synthesised and provided by Tib-Molbiol (Berlin, Germany). |
| T45 |
624-782 |
Sentence |
denotes |
Thermal cycling was performed at 55 °C for 10 min for reverse transcription, followed by 95 °C for 3 min and then 45 cycles of 95 °C for 15 s, 58 °C for 30 s. |
| T46 |
783-926 |
Sentence |
denotes |
Participating laboratories used either Roche Light Cycler 480II or Applied Biosystems ViiA7 instruments (Applied Biosystems, Hong Kong, China). |
