LitCovid-sentences  

PMC:6988269 / 15919-20325 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T134 0-19 Sentence denotes Specificity testing
T135 21-39 Sentence denotes Chemical stability
T136 40-272 Sentence denotes To exclude non-specific reactivity of oligonucleotides among each other, causing artificial fluorescent signals, all assays were tested 120 times in parallel with water and no other nucleic acid except the provided oligonucleotides.
T137 273-333 Sentence denotes In none of these reactions was any positive signal detected.
T138 335-376 Sentence denotes Cross-reactivity with other coronaviruses
T139 377-557 Sentence denotes Cell culture supernatants containing all endemic human coronaviruses (HCoV)‑229E, ‑NL63, ‑OC43 and ‑HKU1 as well as MERS-CoV were tested in duplicate in all three assays (Table 2).
T140 558-639 Sentence denotes For the non-cultivable HCoV-HKU1, supernatant from human airway culture was used.
T141 640-814 Sentence denotes Viral RNA concentration in all samples was determined by specific real-time RT-PCRs and in vitro-transcribed RNA standards designed for absolute quantification of viral load.
T142 815-920 Sentence denotes Additional undiluted (but not quantified) cell culture supernatants were tested as summarised in Table 2.
T143 921-986 Sentence denotes These were additionally mixed into negative human sputum samples.
T144 987-1069 Sentence denotes None of the tested viruses or virus preparations showed reactivity with any assay.
T145 1070-1256 Sentence denotes Table 2 Tests of known respiratory viruses and bacteria in clinical samples and cell culture preparations for cross-reactivity in 2019 novel coronavirus E and RdRp gene assays (n = 310)
T146 1257-1328 Sentence denotes Clinical samples with known viruses Clinical samplesa Virus isolatesb
T147 1329-1346 Sentence denotes HCoV-HKU1 14 1c
T148 1347-1364 Sentence denotes HCoV-OC43 16 2d
T149 1365-1382 Sentence denotes HCoV-NL63 14 1e
T150 1383-1400 Sentence denotes HCoV-229E 18 2f
T151 1401-1416 Sentence denotes MERS-CoV 5 1g
T152 1417-1446 Sentence denotes Influenza A(H1N1)pdm09 17 1
T153 1447-1471 Sentence denotes Influenza A(H3N2) 16 1
T154 1472-1502 Sentence denotes Influenza A (untyped) 11 NA
T155 1503-1526 Sentence denotes Influenza A(H5N1) 1 1
T156 1527-1550 Sentence denotes Influenza A(H7N9) 0 1
T157 1551-1592 Sentence denotes Influenza B (Victoria or Yamagata) 31 1
T158 1593-1623 Sentence denotes Rhinovirus/enterovirus 31 NA
T159 1624-1665 Sentence denotes Respiratory syncytial virus (A/B) 33 NA
T160 1666-1695 Sentence denotes Parainfluenza 1 virus 12 NA
T161 1696-1725 Sentence denotes Parainfluenza 2 virus 11 NA
T162 1726-1755 Sentence denotes Parainfluenza 3 virus 14 NA
T163 1756-1785 Sentence denotes Parainfluenza 4 virus 11 NA
T164 1786-1815 Sentence denotes Human metapneumovirus 16 NA
T165 1816-1833 Sentence denotes Adenovirus 13 1
T166 1834-1856 Sentence denotes Human bocavirus 6 NA
T167 1857-1872 Sentence denotes Legionella spp.
T168 1874-1879 Sentence denotes 3 NA
T169 1880-1895 Sentence denotes Mycoplasma spp.
T170 1897-1902 Sentence denotes 4 NA
T171 1903-1935 Sentence denotes Total clinical samples 297 NA
T172 1936-2069 Sentence denotes a For samples with multiple viruses detected, the virus with highest concentration is listed, as indicated by real-time PCR Ct value.
T173 2070-2135 Sentence denotes b Directly quantified or spiked in human negative-testing sputum.
T174 2136-2201 Sentence denotes c 1 × 105 RNA copies/mL, determined by specific real-time RT-PCR.
T175 2202-2248 Sentence denotes Isolated from human airway epithelial culture.
T176 2249-2330 Sentence denotes d 1 × 1010 RNA copies/mL, determined by specific real-time RT-PCR of one isolate.
T177 2331-2412 Sentence denotes The other isolate was not quantified but spiked in human negative-testing sputum.
T178 2413-2478 Sentence denotes e 4 × 109 RNA copies/mL, determined by specific real-time RT-PCR.
T179 2479-2559 Sentence denotes f 3 × 109 RNA copies/mL, determined by specific real-time RT-PCR of one isolate.
T180 2560-2637 Sentence denotes The other isolate was not quantified spiked in human negative-testing sputum.
T181 2638-2703 Sentence denotes g 1 × 108 RNA copies/mL, determined by specific real-time RT-PCR.
T182 2705-2818 Sentence denotes Exclusivity of 2019 novel coronavirus based on clinical samples pre-tested positive for other respiratory viruses
T183 2819-3080 Sentence denotes Using the E and RdRp gene assays, we tested a total of 297 clinical samples from patients with respiratory disease from the biobanks of five laboratories that provide diagnostic services (one in Germany, two in the Netherlands, one in Hong Kong, one in the UK).
T184 3081-3295 Sentence denotes We selected 198 samples from three university medical centres where patients from general and intensive care wards as well as mainly paediatric outpatient departments are seen (Germany, the Netherlands, Hong Kong).
T185 3296-3462 Sentence denotes The remaining samples were contributed by national public health services performing surveillance studies (RIVM, PHE), with samples mainly submitted by practitioners.
T186 3463-3662 Sentence denotes The samples contained the broadest range of respiratory agents possible and reflected the general spectrum of virus concentrations encountered in diagnostic laboratories in these countries (Table 2).
T187 3663-3721 Sentence denotes In total, this testing yielded no false positive outcomes.
T188 3722-3848 Sentence denotes In four individual test reactions, weak initial reactivity was seen but they were negative upon retesting with the same assay.
T189 3849-4085 Sentence denotes These signals were not associated with any particular virus, and for each virus with which initial positive reactivity occurred, there were other samples that contained the same virus at a higher concentration but did not test positive.
T190 4086-4406 Sentence denotes Given the results from the extensive technical qualification described above, it was concluded that this initial reactivity was not due to chemical instability of real-time PCR probes but most probably to handling issues caused by the rapid introduction of new diagnostic tests and controls during this evaluation study.