| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T119 |
0-98 |
Sentence |
denotes |
Sensitivity based on in vitro-transcribed RNA identical to 2019 novel coronavirus target sequences |
| T120 |
99-384 |
Sentence |
denotes |
Although both assays detected 2019-nCoV without polymorphisms at oligonucleotide binding sites (Figure 2), we additionally generated in vitro-transcribed RNA standards that exactly matched the sequence of 2019-nCoV for absolute quantification and studying the limit of detection (LOD). |
| T121 |
385-511 |
Sentence |
denotes |
Replicate reactions were done at concentrations around the detection end point determined in preliminary dilution experiments. |
| T122 |
512-665 |
Sentence |
denotes |
The resulting LOD from replicate tests was 3.9 copies per reaction for the E gene assay and 3.6 copies per reaction for the RdRp assay (Figure 3C and D). |
| T123 |
666-821 |
Sentence |
denotes |
These figures were close to the 95% hit rate of 2.9 copies per reaction, according to the Poisson distribution, expected when one RNA molecule is detected. |
