LitCovid-sentences  

PMC:6988269 / 11239-12672 JSONTXT 12 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T92 0-171 Sentence denotes To obtain a preliminary assessment of analytical sensitivity, we used purified cell culture supernatant containing SARS-CoV strain Frankfurt-1 virions grown on Vero cells.
T93 172-284 Sentence denotes The supernatant was ultrafiltered and thereby concentrated from a ca 20-fold volume of cell culture supernatant.
T94 285-424 Sentence denotes The concentration step simultaneously reduces the relative concentration of background nucleic acids such as not virion-packaged viral RNA.
T95 425-584 Sentence denotes The virion preparation was quantified by real-time RT-PCR using a specific in vitro-transcribed RNA quantification standard as described in Drosten et al. [8].
T96 585-743 Sentence denotes All assays were subjected to replicate testing in order to determine stochastic detection frequencies at each assay’s sensitivity end point (Figure 3A and B).
T97 744-918 Sentence denotes All assays were highly sensitive, with best results obtained for the E gene and RdRp gene assays (5.2 and 3.8 copies per reaction at 95% detection probability, respectively).
T98 919-971 Sentence denotes These two assays were chosen for further evaluation.
T99 972-1176 Sentence denotes One of the laboratories participating in the external evaluation used other basic RT-PCR reagents (TaqMan Fast Virus 1-Step Master Mix) and repeated the sensitivity study, with equivalent results (E gene:
T100 1177-1209 Sentence denotes 3.2 RNA copies/reaction (95% CI:
T101 1210-1225 Sentence denotes 2.2–6.8); RdRP:
T102 1226-1258 Sentence denotes 3.7 RNA copies/reaction (95% CI:
T103 1259-1268 Sentence denotes 2.8–8.0).
T104 1269-1433 Sentence denotes Of note, the N gene assay also performed well but was not subjected to intensive further validation because it was slightly less sensitive (Supplementary Figure S2)