| Id |
Subject |
Object |
Predicate |
Lexical cue |
| TextSentencer_T1 |
0-98 |
Sentence |
denotes |
Coronavirus endoribonuclease targets viral polyuridine sequences to evade activating host sensors. |
| TextSentencer_T2 |
99-260 |
Sentence |
denotes |
Coronaviruses (CoVs) are positive-sense RNA viruses that can emerge from endemic reservoirs and infect zoonotically, causing significant morbidity and mortality. |
| TextSentencer_T3 |
261-425 |
Sentence |
denotes |
CoVs encode an endoribonuclease designated EndoU that facilitates evasion of host pattern recognition receptor MDA5, but the target of EndoU activity was not known. |
| TextSentencer_T4 |
426-582 |
Sentence |
denotes |
Here, we report that EndoU cleaves the 5'-polyuridines from negative-sense viral RNA, termed PUN RNA, which is the product of polyA-templated RNA synthesis. |
| TextSentencer_T5 |
583-742 |
Sentence |
denotes |
Using a virus containing an EndoU catalytic-inactive mutation, we detected a higher abundance of PUN RNA in the cytoplasm compared to wild-type-infected cells. |
| TextSentencer_T6 |
743-937 |
Sentence |
denotes |
Furthermore, we found that transfecting PUN RNA into cells stimulates a robust, MDA5-dependent interferon response, and that removal of the polyuridine extension on the RNA dampens the response. |
| TextSentencer_T7 |
938-1064 |
Sentence |
denotes |
Overall, the results of this study reveal the PUN RNA to be a CoV MDA5-dependent pathogen-associated molecular pattern (PAMP). |
| TextSentencer_T8 |
1065-1164 |
Sentence |
denotes |
We also establish a mechanism for EndoU activity to cleave and limit the accumulation of this PAMP. |
| TextSentencer_T9 |
1165-1344 |
Sentence |
denotes |
Since EndoU activity is highly conserved in all CoVs, inhibiting this activity may serve as an approach for therapeutic interventions against existing and emerging CoV infections. |
