LitCovid-sample-sentences  

PMC:7443692 / 68246-70536 JSONTXT 10 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T451 0-65 Sentence denotes Analysis of SARS-CoV-2 Spike VSV Pseudoparticles (ppVSV-SARS-2-S)
T452 66-173 Sentence denotes 293T cells were transfected with an expression plasmid encoding SARS-CoV-2 Spike (pcDNAintron-SARS-2-SΔ19).
T453 174-286 Sentence denotes To increase cell surface expression, the last 19 amino acids containing the Golgi retention signal were removed.
T454 287-368 Sentence denotes Two SΔ19 constructs were compared, one started with Met1 and the other with Met2.
T455 369-502 Sentence denotes Twenty-four h following transfection, cells were transduced with ppVSVΔG-VSV-G (particles that were pseudotyped with VSV-G in trans).
T456 503-585 Sentence denotes One h following transduction cells were extensively washed and media was replaced.
T457 586-700 Sentence denotes Supernatant containing particles were collected 12-24 h following transduction and cleared through centrifugation.
T458 701-756 Sentence denotes Cleared supernatant was frozen at −80°C for future use.
T459 757-854 Sentence denotes Target cells Vero E6 were seeded in 24-well plates (5x105 cells/mL) at a density of 80% coverage.
T460 855-1044 Sentence denotes The following day, ppVSV-SARS-2-S/GFP particles were transduced into target cells for 60 min, particles pseudotyped with VSV-G, Lassa virus GP, or no glycoprotein were included as controls.
T461 1045-1265 Sentence denotes 24 h following transduction, transduced cells were released from the plate with trypsin, fixed with 4% formaldehyde, and GFP-positive virus-transduced cells were quantified using flow cytometry (Bectin Dickson BD-LSRII).
T462 1266-1502 Sentence denotes To quantify the ability of various SARS-CoV-2 S mutants to mediate fusion, effector cells (HEK293T) were transiently transfected with the indicated pcDNAintron-SARS-2-S expression vector or measles virus H and F (Brindley et al., 2014).
T463 1503-1623 Sentence denotes Effector cells were infected with MVA-T7 four h following transduction to produce the T7 polymerase (Paal et al., 2009).
T464 1624-1856 Sentence denotes Target cells naturally expressing the receptor ACE2 (Vero) or ACE2 negative cells (HEK293T) were transfected with pTM1-luciferase, which encodes for firefly luciferase under the control of a T7 promoter (Brindley and Plemper, 2010).
T465 1857-1962 Sentence denotes 24 h following transfection, the target cells were lifted and added to the effector cells at a 1:1 ratio.
T466 1963-2093 Sentence denotes 4 h following co-cultivation, cells were washed, lysed and luciferase levels were quantified using Promega’s Steady-Glo substrate.
T467 2094-2209 Sentence denotes To visualize cell-to-cell fusion, Vero cells were co-transfected with pGFP and the pcDNAintron-SARS-2-S constructs.
T468 2210-2290 Sentence denotes 24 h following transfection, syncytia was visualized by fluorescence microscopy.