LitCovid-sample-sentences  

PMC:7443692 / 53649-55061 JSONTXT 9 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T366 0-61 Sentence denotes Analysis of Disulfide Bonds for SARS-Cov-2 S Protein by LC-MS
T367 62-247 Sentence denotes Two 10-μg aliquots of SARS-CoV-2 S protein were denatured by incubating with 20% acetonitrile at room temperature and alkylated by 13.75 mM of iodoacetamide at room temperature in dark.
T368 248-382 Sentence denotes The two aliquots of proteins were then digested respectively using alpha lytic protease, or a combination of trypsin, Lys-C and Glu-C.
T369 383-458 Sentence denotes Following digestion, the proteins were deglycosylated by PNGaseF treatment.
T370 459-682 Sentence denotes The resulting peptides were separated on an Acclaim PepMap RSLC C18 column (75 μm x 15 cm) and eluted into the nano-electrospray ion source of an Orbitrap Fusion Lumos Tribrid mass spectrometer at a flow rate of 200 nL/min.
T371 683-828 Sentence denotes The elution gradient consists of 1%–40% acetonitrile in 0.1% formic acid over 370 min followed by 10 min of 80% acetonitrile in 0.1% formic acid.
T372 829-926 Sentence denotes The spray voltage was set to 2.2 kV and the temperature of the heated capillary was set to 280°C.
T373 927-1107 Sentence denotes Full MS scans were acquired from m/z 200 to 2000 at 60k resolution, and MS/MS scans following electron transfer dissociation (ETD) were collected in the Orbitrap at 15k resolution.
T374 1108-1248 Sentence denotes The raw spectra were analyzed by Byonic (v3.8.13, Protein Metrics Inc.) with mass tolerance set as 20 ppm for both precursors and fragments.
T375 1249-1329 Sentence denotes The search output was filtered at 1% false discovery rate and 10 ppm mass error.
T376 1330-1412 Sentence denotes The spectra assigned as cross-linked peptides were manually evaluated for Cys0015.