LitCovid-sample-sentences  

PMC:7443692 / 48615-50316 JSONTXT 8 Projects

Annnotations TAB TSV DIC JSON TextAE

Id Subject Object Predicate Lexical cue
T332 0-427 Sentence denotes To express a stabilized ectodomain of Spike protein, a synthetic gene encoding residues 1−1208 of SARS-CoV-2 Spike with the furin cleavage site (residues 682–685) replaced by a “GGSG” sequence, proline substitutions at residues 986 and 987, and a foldon trimerization motif followed by a C-terminal 6xHisTag was created and cloned into the mammalian expression vector pCMV-IRES-puro (Codex BioSolutions, Inc, Gaithersburg, MD).
T333 428-557 Sentence denotes The expression construct was transiently transfected in HEK293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA).
T334 558-802 Sentence denotes Protein was purified from cell supernatants using Ni-NTA resin (QIAGEN, Germany), the eluted fractions containing S protein were pooled, concentrated, and further purified by gel filtration chromatography on a Superose 6 column (GE Healthcare).
T335 803-900 Sentence denotes Negative stain electron microscopy (EM) analysis was performed as described (Shaik et al., 2019).
T336 901-1194 Sentence denotes Briefly, analysis was performed at room temperature with a magnification of 52,000x and a defocus value of 1.5 μm following low-dose procedures, using a Philips Tecnai F20 electron microscope (Thermo Fisher Scientific) equipped with a Gatan US4000 CCD camera and operated at voltage of 200 kV.
T337 1195-1340 Sentence denotes The DNA fragment encoding human ACE2 (1-615) with a 6xHis tag at C terminus was synthesized by Genscript and cloned to the vector pCMV-IRES-puro.
T338 1341-1422 Sentence denotes The expression construct was transfected in HEK293F cells using polyethylenimine.
T339 1423-1499 Sentence denotes The medium was discarded and replaced with FreeStyle 293 medium after 6-8 h.
T340 1500-1625 Sentence denotes After incubation in 37°C with 5.5% CO2 for 5 days, the supernatant was collected and loaded to Ni-NTA resin for purification.
T341 1626-1701 Sentence denotes The elution was concentrated and further purified by a Superdex 200 column.