| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T162 |
0-118 |
Sentence |
denotes |
We also analyzed ACE2 glycosylation utilizing the same glycomic and glycoproteomic approaches described for S protein. |
| T163 |
119-348 |
Sentence |
denotes |
Glycomic analyses of released N-linked glycans (Figure 5 A; Table S3) revealed that the majority of glycans on ACE2 are complex with limited high-mannose and hybrid glycans, and we were unable to detect sulfated N-linked glycans. |
| T164 |
349-555 |
Sentence |
denotes |
Glycoproteomic analyses revealed that occupancy was high (> 75%) at all six sites, and significant microheterogeneity dominated by complex N-glycans was observed for each site (Figures 5B–5G; Tables S5–S8). |
| T165 |
556-816 |
Sentence |
denotes |
We also observed, consistent with the O-glycomics (Figure S5; Table S4), that Ser 155 and several S/T residues at the C terminus of ACE2 outside of the peptidase domain were O-glycosylated, but stoichiometry was extremely low (less than 2%; Tables S9 and S10). |
| T166 |
817-937 |
Sentence |
denotes |
Figure 5 Glycomics-Informed Glycoproteomics of Soluble Human ACE2 Reveals High Occupancy, Complex N-linked Glycosylation |
| T167 |
938-1026 |
Sentence |
denotes |
(A) Glycans released from soluble, purified ACE2 were permethylated and analyzed by MSn. |
| T168 |
1027-1145 |
Sentence |
denotes |
Structures were assigned, grouped by type and structural features, and prevalence was determined based on ion current. |
| T169 |
1146-1204 |
Sentence |
denotes |
The pie chart shows basic division by broad N-glycan type. |
| T170 |
1205-1273 |
Sentence |
denotes |
The bar graph provides additional detail about the glycans detected. |
| T171 |
1274-1447 |
Sentence |
denotes |
The most abundant structure with a unique categorization by glycomics for each N-glycan type in the pie chart, or above each feature category in the bar graph, is indicated. |
| T172 |
1448-1653 |
Sentence |
denotes |
(B–G) Glycopeptides were prepared from soluble human ACE2 by using multiple combinations of proteases, analyzed by LC-MSn, and the resulting data were searched by using several different software packages. |
| T173 |
1654-1713 |
Sentence |
denotes |
All six sites of N-linked glycosylation are presented here. |
| T174 |
1714-1828 |
Sentence |
denotes |
Displayed in the bar graphs are the individual compositions observed graphed in terms of assigned spectral counts. |
| T175 |
1829-1949 |
Sentence |
denotes |
Representative glycans (as determined by glycomics analysis) for several abundant compositions are shown in SNFG format. |
| T176 |
1950-2066 |
Sentence |
denotes |
The pie chart (analogous to Figure 3 for SARS-CoV-2 S) for each site is displayed in the upper corner of each panel. |
| T177 |
2067-2076 |
Sentence |
denotes |
(B) N053. |
| T178 |
2077-2086 |
Sentence |
denotes |
(C) N090. |
| T179 |
2087-2096 |
Sentence |
denotes |
(D) N103. |
| T180 |
2097-2106 |
Sentence |
denotes |
(E) N322. |
| T181 |
2107-2116 |
Sentence |
denotes |
(F) N432. |
| T182 |
2117-2180 |
Sentence |
denotes |
(G) N546, a site that does not exist in three in 10,000 people. |
