| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T161 |
0-81 |
Sentence |
denotes |
Glycomics-Informed Glycoproteomics Reveals Complex N-linked Glycosylation of ACE2 |
| T162 |
82-200 |
Sentence |
denotes |
We also analyzed ACE2 glycosylation utilizing the same glycomic and glycoproteomic approaches described for S protein. |
| T163 |
201-430 |
Sentence |
denotes |
Glycomic analyses of released N-linked glycans (Figure 5 A; Table S3) revealed that the majority of glycans on ACE2 are complex with limited high-mannose and hybrid glycans, and we were unable to detect sulfated N-linked glycans. |
| T164 |
431-637 |
Sentence |
denotes |
Glycoproteomic analyses revealed that occupancy was high (> 75%) at all six sites, and significant microheterogeneity dominated by complex N-glycans was observed for each site (Figures 5B–5G; Tables S5–S8). |
| T165 |
638-898 |
Sentence |
denotes |
We also observed, consistent with the O-glycomics (Figure S5; Table S4), that Ser 155 and several S/T residues at the C terminus of ACE2 outside of the peptidase domain were O-glycosylated, but stoichiometry was extremely low (less than 2%; Tables S9 and S10). |
| T166 |
899-1019 |
Sentence |
denotes |
Figure 5 Glycomics-Informed Glycoproteomics of Soluble Human ACE2 Reveals High Occupancy, Complex N-linked Glycosylation |
| T167 |
1020-1108 |
Sentence |
denotes |
(A) Glycans released from soluble, purified ACE2 were permethylated and analyzed by MSn. |
| T168 |
1109-1227 |
Sentence |
denotes |
Structures were assigned, grouped by type and structural features, and prevalence was determined based on ion current. |
| T169 |
1228-1286 |
Sentence |
denotes |
The pie chart shows basic division by broad N-glycan type. |
| T170 |
1287-1355 |
Sentence |
denotes |
The bar graph provides additional detail about the glycans detected. |
| T171 |
1356-1529 |
Sentence |
denotes |
The most abundant structure with a unique categorization by glycomics for each N-glycan type in the pie chart, or above each feature category in the bar graph, is indicated. |
| T172 |
1530-1735 |
Sentence |
denotes |
(B–G) Glycopeptides were prepared from soluble human ACE2 by using multiple combinations of proteases, analyzed by LC-MSn, and the resulting data were searched by using several different software packages. |
| T173 |
1736-1795 |
Sentence |
denotes |
All six sites of N-linked glycosylation are presented here. |
| T174 |
1796-1910 |
Sentence |
denotes |
Displayed in the bar graphs are the individual compositions observed graphed in terms of assigned spectral counts. |
| T175 |
1911-2031 |
Sentence |
denotes |
Representative glycans (as determined by glycomics analysis) for several abundant compositions are shown in SNFG format. |
| T176 |
2032-2148 |
Sentence |
denotes |
The pie chart (analogous to Figure 3 for SARS-CoV-2 S) for each site is displayed in the upper corner of each panel. |
| T177 |
2149-2158 |
Sentence |
denotes |
(B) N053. |
| T178 |
2159-2168 |
Sentence |
denotes |
(C) N090. |
| T179 |
2169-2178 |
Sentence |
denotes |
(D) N103. |
| T180 |
2179-2188 |
Sentence |
denotes |
(E) N322. |
| T181 |
2189-2198 |
Sentence |
denotes |
(F) N432. |
| T182 |
2199-2262 |
Sentence |
denotes |
(G) N546, a site that does not exist in three in 10,000 people. |
