Id |
Subject |
Object |
Predicate |
Lexical cue |
T85 |
0-138 |
Sentence |
denotes |
Figure 2 Glycomics-Informed Glycoproteomics Reveals Substantial Site-Specific Microheterogeneity of N-linked Glycosylation on SARS-CoV-2 S |
T86 |
139-242 |
Sentence |
denotes |
(A) Glycans released from SARS-CoV-2 S protein trimer immunogen were permethylated and analyzed by MSn. |
T87 |
243-364 |
Sentence |
denotes |
Structures were assigned and grouped by type and structural features, and prevalence was determined based on ion current. |
T88 |
365-423 |
Sentence |
denotes |
The pie chart shows basic division by broad N-glycan type. |
T89 |
424-492 |
Sentence |
denotes |
The bar graph provides additional detail about the glycans detected. |
T90 |
493-666 |
Sentence |
denotes |
The most abundant structure with a unique categorization by glycomics for each N-glycan type in the pie chart, or above each feature category in the bar graph, is indicated. |
T91 |
667-891 |
Sentence |
denotes |
(B–E) Glycopeptides were prepared from SARS-CoV-2 S protein trimer immunogen by using multiple combinations of proteases, analyzed by LC-MSn, and the resulting data were searched by using several different software packages. |
T92 |
892-1014 |
Sentence |
denotes |
Four representative sites of N-linked glycosylation with specific features of interest were chosen and are presented here. |
T93 |
1015-1243 |
Sentence |
denotes |
N0074 (B) and N0149 (C) are shown that occur in variable insert regions of S compared to SARS-CoV and other related coronaviruses, and there are emerging variants of SARS-CoV-2 that disrupt these two sites of glycosylation in S. |
T94 |
1244-1302 |
Sentence |
denotes |
N0234 (D) contains the most high-mannose N-linked glycans. |
T95 |
1303-1453 |
Sentence |
denotes |
N0801 (D) is an example of glycosylation in the S2 region of the immunogen and displays a high degree of hybrid glycosylation compared to other sites. |
T96 |
1454-1536 |
Sentence |
denotes |
The abundance of each composition is graphed in terms of assigned spectral counts. |
T97 |
1537-1657 |
Sentence |
denotes |
Representative glycans (as determined by glycomics analysis) for several abundant compositions are shown in SNFG format. |
T98 |
1658-1789 |
Sentence |
denotes |
The abbreviations used here and throughout the manuscript are as follows: N, HexNAc; H, hexose; F, fucose; A, Neu5Ac; S, sulfation. |
T99 |
1790-1954 |
Sentence |
denotes |
Note that the graphs for the other 18 sites and other graphs grouping the microheterogeneity observed by other properties are presented in Supplemental Information. |