Id |
Subject |
Object |
Predicate |
Lexical cue |
T66 |
0-169 |
Sentence |
denotes |
(D) A representative Step-HCD fragmentation spectrum from mass-spectrometry analysis of a tryptic digest of S annotated manually based on search results from pGlyco 2.2. |
T67 |
170-262 |
Sentence |
denotes |
This spectrum defines the N terminus of the mature protein monomer as (pyro-)glutamine 0014. |
T68 |
263-424 |
Sentence |
denotes |
A representative N-glycan consistent with this annotation and our glycomics data (Figure 2) is overlaid by using the Symbol Nomenclature For Glycans (SNFG) code. |
T69 |
425-461 |
Sentence |
denotes |
This complex glycan occurs at N0017. |
T70 |
462-581 |
Sentence |
denotes |
Note, that as expected, the cysteine is carbamidomethylated, and the mass accuracy of the assigned peptide is 0.98 ppm. |
T71 |
582-694 |
Sentence |
denotes |
On the sequence of the N-terminal peptide and in the spectrum, the assigned b (blue) and y (red) ions are shown. |
T72 |
695-848 |
Sentence |
denotes |
In the spectrum, purple highlights glycan oxonium ions and green marks intact peptide fragment ions with various partial glycan sequences still attached. |
T73 |
849-1016 |
Sentence |
denotes |
Note that the green-labeled ions allow for limited topology to be extracted including defining that the fucose is on the core and not the antennae of the glycopeptide. |