LitCovid-sample-sentences  

PMC:7253482 / 5594-8420 JSONTXT 23 Projects

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Id Subject Object Predicate Lexical cue
T32 0-212 Sentence denotes To generate a soluble mimic of the viral S proteins, we used the 2P-stabilised native-like SARS and MERS S protein antigens, the design and structures of which have been described previously by Pallesen et al.41.
T33 213-316 Sentence denotes SARS, MERS and HKU1 S genes encode many N-linked glycan sequons; 23, 23 and 29, respectively (Fig. 1a).
T34 317-442 Sentence denotes We initially sought to quantitatively assess the composition of the carbohydrate structures displayed on the S glycoproteins.
T35 443-622 Sentence denotes N-linked glycans were enzymatically released, fluorescently labelled, and subjected to hydrophilic interaction chromatography-ultra-performance liquid chromatography (HILIC-UPLC).
T36 623-778 Sentence denotes Treatment with endoglycosidase H (Endo H) revealed a population (SARS 32.2%; MERS 33.8%, HKU1 25.0%) of underprocessed oligomannose-type glycans (Fig. 1b).
T37 779-983 Sentence denotes This observation of both complex and oligomannose-type glycans reveals that the majority of N-linked glycans can be processed, although there is limited processing at specific sites across the S proteins.
T38 984-1233 Sentence denotes It is also interesting to note that the distribution of oligomannose-type glycans was broad, with Man5GlcNAc2 to Man9GlcNAc2 glycans all present, without one particular dominant peak, as is the case for some viral glycoproteins, such as HIV-1 Env36.
T39 1234-1423 Sentence denotes The proportion of oligomannose-type glycans on recombinant coronavirus S proteins is consistent with previous studies performed on virally derived MERS and SARS coronavirus S proteins17,42.
T40 1424-1585 Sentence denotes Coronaviruses have been previously been reported to form virions by budding into the lumen of endoplasmic reticulum-Golgi intermediate compartments (ERGIC)43,44.
T41 1586-1908 Sentence denotes Observations of hybrid- and complex-type glycans on virally derived material17,42 would, however, suggest that it is likely that coronavirus virions travel through the Golgi apparatus after virion formation in the ERGIC en route to the cell surface, thus supporting recombinant immunogens as models of viral glycoproteins.
T42 1909-1970 Sentence denotes Fig. 1 Compositional analysis of SARS, MERS and HKU1 glycans.
T43 1971-2156 Sentence denotes a Schematic representation of SARS, MERS and HKU1 coronavirus S glycoproteins, showing the positions of N-linked glycosylation amino-acid sequons (NXS/T, where X ≠ P) shown as branches.
T44 2157-2485 Sentence denotes The domains of the S glycoproteins are illustrated: N-terminal domain (NTD), receptor-binding domain (RBD), sub-domain 1/2 (SD1/2), fusion peptide (FP), heptad repeat 1/2 (HR1/2), central helix (CH), and transmembrane domain (TM). b HILIC-UPLC chromatograms of fluorescently labelled N-linked glycans from SARS, MERS and HKU1 S.
T45 2486-2696 Sentence denotes Oligomannose-type glycans (M5 to M9; Man5GlcNAc2–Man9GlcNAc2) (green) and complex-type glycans (magenta) were identified by Endo H digestion, with quantification of major glycan types summarised as a pie chart.
T46 2697-2826 Sentence denotes Oligomannose-type glycans are schematically annotated with mannose residues as green circles and GlcNAc residues as blue squares.