| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T151 |
0-62 |
Sentence |
denotes |
Expression and purification of coronavirus spike glycoproteins |
| T152 |
63-341 |
Sentence |
denotes |
Human embryonic kidney 293 Freestyle (HEK293F) cells were transfected with mammalian-codon-optimised genes encoding 2P-stabilised SARS MERS and HKU1 S proteins containing a C-terminal T4 fibritin trimerization domain, an HRV3C cleavage site, an 8xHis-tag and a Twin-Strep-tag41. |
| T153 |
342-415 |
Sentence |
denotes |
H3N2 Victoria 2011 hemagglutinin was also expressed in the HEK293F cells. |
| T154 |
416-623 |
Sentence |
denotes |
The 200 ml cultures were harvested 6 days after transfection, filtered and purified by nickel-affinity chromatography and size exclusion chromatography using a SuperdexTM 16/600 75 pg column (GE Healthcare). |
