| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T116 |
0-121 |
Sentence |
denotes |
4.1.3.1 Hypothesis: SARS-CoV and SARS-CoV2 are ideally similar in the structure and the cell entry receptor and protease |
| T117 |
122-484 |
Sentence |
denotes |
SARS-CoV and SARS-CoV2 share absolutely the same cleavage junctions, almost the same sequence (96%) of their main protease, a high degree (76%) of similarity in the amino acid sequence of their S protein, a similar S2′ cleavage site, a similar spectrum of cells they can enter, and the similarity of the most residues essential for binding ACE2 [16], [17], [18]. |
| T118 |
485-570 |
Sentence |
denotes |
Also, both of them utilize the same domain of S1B to interact with the ACE2 receptor. |
| T119 |
571-633 |
Sentence |
denotes |
However, they differ in proteolytic processing to some degree. |
| T120 |
634-828 |
Sentence |
denotes |
Study [16] of the human embryonic kidney (HEK) cell line, 293 T, has shown that a signal for the S2 subunit is present in cells inoculated with SARS-2-S, but not in cells inoculated with SARS-S. |
| T121 |
829-990 |
Sentence |
denotes |
Two main proteases for both SARS-S and SARS-2-S are endosomal cysteine proteases cathepsin B and L (CatB/L) and the transmembrane protease, serine 2TMPRSS2 [16]. |
| T122 |
991-1170 |
Sentence |
denotes |
In 293 T cells lacking 2TMPRSS2, blocking CatB/L activity through increasing the endosomal pH by ammonium chloride could significantly limit the entry of both SARS-S and SARS-2-S. |
| T123 |
1171-1257 |
Sentence |
denotes |
In TMPRSS2 + Caco-2 cells, the effect of ammonium chloride existed to a lesser extent. |
| T124 |
1258-1427 |
Sentence |
denotes |
A combination of camostat mesylate, a blocker of TMPRSS2, and E-64d, an inhibitor of CatB/L, yielded the complete inhibition of SARS-2-S entry in TMPRSS2 + Caco-2 cells. |
| T125 |
1428-1677 |
Sentence |
denotes |
In both the human lung cancer cell line Calu-3 and the primary human lung cells, there was a reduction of the entry of both SARS-S and SARS-2-S by camostat mesylate, indicating that SARS-S and SARS-2-S partially require TMPRSS2 for a lung infection. |
