| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T33 |
0-418 |
Sentence |
denotes |
To resolve the site-specific glycosylation of the SARS-CoV-2 S protein and visualize the distribution of glycoforms across the protein surface, we expressed and purified three biological replicates of recombinant soluble material in an identical manner to that which was used to obtain the high-resolution cryo–electron microscopy (cryo-EM) structure, albeit without a glycan-processing blockade using kifunensine (4). |
| T34 |
419-511 |
Sentence |
denotes |
This variant of the S protein contains all 22 glycans on the SARS-CoV-2 S protein (Fig. 1A). |
| T35 |
512-768 |
Sentence |
denotes |
Stabilization of the trimeric prefusion structure was achieved by using the 2P stabilizing mutations (20) at residues 986 and 987, a GSAS (Gly-Ser-Ala-Ser) substitution at the furin cleavage site (residues 682 to 685), and a C-terminal trimerization motif. |
| T36 |
769-841 |
Sentence |
denotes |
This helps to maintain quaternary architecture during glycan processing. |
| T37 |
842-1041 |
Sentence |
denotes |
Before analysis, supernatant containing the recombinant SARS-CoV-2 S was purified by size exclusion chromatography to ensure that only native-like trimeric protein was analyzed (Fig. 1B and fig. S1). |
| T38 |
1042-1144 |
Sentence |
denotes |
The trimeric conformation of the purified material was validated by using negative-stain EM (Fig. 1C). |
