Id |
Subject |
Object |
Predicate |
Lexical cue |
T671 |
0-2 |
TO |
denotes |
To |
T672 |
3-10 |
VB |
denotes |
resolve |
T673 |
11-14 |
DT |
denotes |
the |
T674 |
15-28 |
JJ |
denotes |
site-specific |
T675 |
29-42 |
NN |
denotes |
glycosylation |
T676 |
43-45 |
IN |
denotes |
of |
T677 |
46-49 |
DT |
denotes |
the |
T678 |
50-60 |
NN |
denotes |
SARS-CoV-2 |
T679 |
61-62 |
NN |
denotes |
S |
T680 |
63-70 |
NN |
denotes |
protein |
T681 |
71-74 |
CC |
denotes |
and |
T682 |
75-84 |
VB |
denotes |
visualize |
T683 |
85-88 |
DT |
denotes |
the |
T684 |
89-101 |
NN |
denotes |
distribution |
T685 |
102-104 |
IN |
denotes |
of |
T686 |
105-115 |
NNS |
denotes |
glycoforms |
T687 |
116-122 |
IN |
denotes |
across |
T688 |
123-126 |
DT |
denotes |
the |
T689 |
127-134 |
NN |
denotes |
protein |
T690 |
135-142 |
NN |
denotes |
surface |
T691 |
142-143 |
-COMMA- |
denotes |
, |
T692 |
144-146 |
PRP |
denotes |
we |
T693 |
147-156 |
VBD |
denotes |
expressed |
T694 |
157-160 |
CC |
denotes |
and |
T695 |
161-169 |
VBN |
denotes |
purified |
T696 |
170-175 |
CD |
denotes |
three |
T697 |
176-186 |
JJ |
denotes |
biological |
T698 |
187-197 |
NNS |
denotes |
replicates |
T699 |
198-200 |
IN |
denotes |
of |
T700 |
201-212 |
JJ |
denotes |
recombinant |
T701 |
213-220 |
JJ |
denotes |
soluble |
T702 |
221-229 |
NN |
denotes |
material |
T703 |
230-232 |
IN |
denotes |
in |
T704 |
233-235 |
DT |
denotes |
an |
T705 |
236-245 |
JJ |
denotes |
identical |
T706 |
246-252 |
NN |
denotes |
manner |
T707 |
253-255 |
TO |
denotes |
to |
T708 |
256-260 |
DT |
denotes |
that |
T709 |
261-266 |
WDT |
denotes |
which |
T710 |
267-270 |
VBD |
denotes |
was |
T711 |
271-275 |
VBN |
denotes |
used |
T712 |
276-278 |
TO |
denotes |
to |
T713 |
279-285 |
VB |
denotes |
obtain |
T714 |
286-289 |
DT |
denotes |
the |
T715 |
290-305 |
JJ |
denotes |
high-resolution |
T716 |
306-319 |
NN |
denotes |
cryo–electron |
T717 |
320-330 |
NN |
denotes |
microscopy |
T718 |
331-332 |
-LRB- |
denotes |
( |
T719 |
332-339 |
NN |
denotes |
cryo-EM |
T720 |
339-340 |
-RRB- |
denotes |
) |
T721 |
341-350 |
NN |
denotes |
structure |
T722 |
350-351 |
-COMMA- |
denotes |
, |
T723 |
352-358 |
IN |
denotes |
albeit |
T724 |
359-366 |
IN |
denotes |
without |
T725 |
367-368 |
DT |
denotes |
a |
T726 |
369-386 |
NN |
denotes |
glycan-processing |
T727 |
387-395 |
NN |
denotes |
blockade |
T728 |
396-401 |
VBG |
denotes |
using |
T729 |
402-413 |
NN |
denotes |
kifunensine |
T730 |
414-415 |
-LRB- |
denotes |
( |
T731 |
415-416 |
CD |
denotes |
4 |
T732 |
416-417 |
-RRB- |
denotes |
) |
T733 |
419-423 |
DT |
denotes |
This |
T734 |
424-431 |
NN |
denotes |
variant |
T735 |
432-434 |
IN |
denotes |
of |
T736 |
435-438 |
DT |
denotes |
the |
T737 |
439-440 |
NN |
denotes |
S |
T738 |
441-448 |
NN |
denotes |
protein |
T739 |
449-457 |
VBZ |
denotes |
contains |
T740 |
458-461 |
DT |
denotes |
all |
T741 |
462-464 |
CD |
denotes |
22 |
T742 |
465-472 |
NNS |
denotes |
glycans |
T743 |
473-475 |
IN |
denotes |
on |
T744 |
476-479 |
DT |
denotes |
the |
T745 |
480-490 |
NN |
denotes |
SARS-CoV-2 |
T746 |
491-492 |
NN |
denotes |
S |
T747 |
493-500 |
NN |
denotes |
protein |
T748 |
501-502 |
-LRB- |
denotes |
( |
T749 |
502-506 |
NN |
denotes |
Fig. |
T750 |
507-509 |
NN |
denotes |
1A |
T751 |
509-510 |
-RRB- |
denotes |
) |
T752 |
512-525 |
NN |
denotes |
Stabilization |
T753 |
526-528 |
IN |
denotes |
of |
T754 |
529-532 |
DT |
denotes |
the |
T755 |
533-541 |
JJ |
denotes |
trimeric |
T756 |
542-551 |
NN |
denotes |
prefusion |
T757 |
552-561 |
NN |
denotes |
structure |
T758 |
562-565 |
VBD |
denotes |
was |
T759 |
566-574 |
VBN |
denotes |
achieved |
T760 |
575-577 |
IN |
denotes |
by |
T761 |
578-583 |
VBG |
denotes |
using |
T762 |
584-587 |
DT |
denotes |
the |
T763 |
588-590 |
NN |
denotes |
2P |
T764 |
591-602 |
VBG |
denotes |
stabilizing |
T765 |
603-612 |
NNS |
denotes |
mutations |
T766 |
613-614 |
-LRB- |
denotes |
( |
T767 |
614-616 |
CD |
denotes |
20 |
T768 |
616-617 |
-RRB- |
denotes |
) |
T769 |
618-620 |
IN |
denotes |
at |
T770 |
621-629 |
NNS |
denotes |
residues |
T771 |
630-633 |
CD |
denotes |
986 |
T772 |
634-637 |
CC |
denotes |
and |
T773 |
638-641 |
CD |
denotes |
987 |
T774 |
641-642 |
-COMMA- |
denotes |
, |
T775 |
643-644 |
DT |
denotes |
a |
T776 |
645-649 |
NN |
denotes |
GSAS |
T777 |
650-651 |
-LRB- |
denotes |
( |
T778 |
651-666 |
NN |
denotes |
Gly-Ser-Ala-Ser |
T779 |
666-667 |
-RRB- |
denotes |
) |
T780 |
668-680 |
NN |
denotes |
substitution |
T781 |
681-683 |
IN |
denotes |
at |
T782 |
684-687 |
DT |
denotes |
the |
T783 |
688-693 |
NN |
denotes |
furin |
T784 |
694-702 |
NN |
denotes |
cleavage |
T785 |
703-707 |
NN |
denotes |
site |
T786 |
708-709 |
-LRB- |
denotes |
( |
T787 |
709-717 |
NNS |
denotes |
residues |
T788 |
718-721 |
CD |
denotes |
682 |
T789 |
722-724 |
TO |
denotes |
to |
T790 |
725-728 |
CD |
denotes |
685 |
T791 |
728-729 |
-RRB- |
denotes |
) |
T792 |
729-730 |
-COMMA- |
denotes |
, |
T793 |
731-734 |
CC |
denotes |
and |
T794 |
735-736 |
DT |
denotes |
a |
T795 |
737-747 |
JJ |
denotes |
C-terminal |
T796 |
748-761 |
NN |
denotes |
trimerization |
T797 |
762-767 |
NN |
denotes |
motif |
T798 |
769-773 |
DT |
denotes |
This |
T799 |
774-779 |
VBZ |
denotes |
helps |
T800 |
780-782 |
TO |
denotes |
to |
T801 |
783-791 |
VB |
denotes |
maintain |
T802 |
792-802 |
JJ |
denotes |
quaternary |
T803 |
803-815 |
NN |
denotes |
architecture |
T804 |
816-822 |
IN |
denotes |
during |
T805 |
823-829 |
NN |
denotes |
glycan |
T806 |
830-840 |
NN |
denotes |
processing |
T807 |
842-848 |
IN |
denotes |
Before |
T808 |
849-857 |
NN |
denotes |
analysis |
T809 |
857-858 |
-COMMA- |
denotes |
, |
T810 |
859-870 |
NN |
denotes |
supernatant |
T811 |
871-881 |
VBG |
denotes |
containing |
T812 |
882-885 |
DT |
denotes |
the |
T813 |
886-897 |
JJ |
denotes |
recombinant |
T814 |
898-908 |
NN |
denotes |
SARS-CoV-2 |
T815 |
909-910 |
NN |
denotes |
S |
T816 |
911-914 |
VBD |
denotes |
was |
T817 |
915-923 |
VBN |
denotes |
purified |
T818 |
924-926 |
IN |
denotes |
by |
T819 |
927-931 |
NN |
denotes |
size |
T820 |
932-941 |
NN |
denotes |
exclusion |
T821 |
942-956 |
NN |
denotes |
chromatography |
T822 |
957-959 |
TO |
denotes |
to |
T823 |
960-966 |
VB |
denotes |
ensure |
T824 |
967-971 |
IN |
denotes |
that |
T825 |
972-976 |
RB |
denotes |
only |
T826 |
977-988 |
JJ |
denotes |
native-like |
T827 |
989-997 |
JJ |
denotes |
trimeric |
T828 |
998-1005 |
NN |
denotes |
protein |
T829 |
1006-1009 |
VBD |
denotes |
was |
T830 |
1010-1018 |
VBN |
denotes |
analyzed |
T831 |
1019-1020 |
-LRB- |
denotes |
( |
T832 |
1020-1024 |
NNP |
denotes |
Fig. |
T833 |
1025-1027 |
NN |
denotes |
1B |
T834 |
1028-1031 |
CC |
denotes |
and |
T835 |
1032-1035 |
NN |
denotes |
fig |
T836 |
1037-1039 |
NN |
denotes |
S1 |
T837 |
1039-1040 |
-RRB- |
denotes |
) |
T838 |
1042-1045 |
DT |
denotes |
The |
T839 |
1046-1054 |
JJ |
denotes |
trimeric |
T840 |
1055-1067 |
NN |
denotes |
conformation |
T841 |
1068-1070 |
IN |
denotes |
of |
T842 |
1071-1074 |
DT |
denotes |
the |
T843 |
1075-1083 |
VBN |
denotes |
purified |
T844 |
1084-1092 |
NN |
denotes |
material |
T845 |
1093-1096 |
VBD |
denotes |
was |
T846 |
1097-1106 |
VBN |
denotes |
validated |
T847 |
1107-1109 |
IN |
denotes |
by |
T848 |
1110-1115 |
VBG |
denotes |
using |
T849 |
1116-1130 |
JJ |
denotes |
negative-stain |
T850 |
1131-1133 |
NN |
denotes |
EM |
T851 |
1134-1135 |
-LRB- |
denotes |
( |
T852 |
1135-1139 |
NN |
denotes |
Fig. |
T853 |
1140-1142 |
NN |
denotes |
1C |
T854 |
1142-1143 |
-RRB- |
denotes |
) |
R673 |
T681 |
T671 |
arg1Of |
and,To |
R674 |
T693 |
T671 |
modOf |
expressed,To |
R675 |
T692 |
T672 |
arg1Of |
we,resolve |
R676 |
T675 |
T672 |
arg2Of |
glycosylation,resolve |
R677 |
T675 |
T673 |
arg1Of |
glycosylation,the |
R678 |
T675 |
T674 |
arg1Of |
glycosylation,site-specific |
R679 |
T675 |
T676 |
arg1Of |
glycosylation,of |
R680 |
T680 |
T676 |
arg2Of |
protein,of |
R681 |
T680 |
T677 |
arg1Of |
protein,the |
R682 |
T680 |
T678 |
arg1Of |
protein,SARS-CoV-2 |
R683 |
T680 |
T679 |
arg1Of |
protein,S |
R684 |
T672 |
T681 |
arg1Of |
resolve,and |
R685 |
T682 |
T681 |
arg2Of |
visualize,and |
R686 |
T692 |
T682 |
arg1Of |
we,visualize |
R687 |
T684 |
T682 |
arg2Of |
distribution,visualize |
R688 |
T684 |
T683 |
arg1Of |
distribution,the |
R689 |
T684 |
T685 |
arg1Of |
distribution,of |
R690 |
T686 |
T685 |
arg2Of |
glycoforms,of |
R691 |
T684 |
T687 |
arg1Of |
distribution,across |
R692 |
T690 |
T687 |
arg2Of |
surface,across |
R693 |
T690 |
T688 |
arg1Of |
surface,the |
R694 |
T690 |
T689 |
arg1Of |
surface,protein |
R695 |
T693 |
T691 |
arg1Of |
expressed,"," |
R696 |
T692 |
T693 |
arg1Of |
we,expressed |
R697 |
T698 |
T693 |
arg2Of |
replicates,expressed |
R698 |
T698 |
T694 |
arg1Of |
replicates,and |
R699 |
T698 |
T695 |
arg2Of |
replicates,purified |
R700 |
T698 |
T696 |
arg1Of |
replicates,three |
R701 |
T698 |
T697 |
arg1Of |
replicates,biological |
R702 |
T698 |
T699 |
arg1Of |
replicates,of |
R703 |
T702 |
T699 |
arg2Of |
material,of |
R704 |
T702 |
T700 |
arg1Of |
material,recombinant |
R705 |
T702 |
T701 |
arg1Of |
material,soluble |
R706 |
T693 |
T703 |
arg1Of |
expressed,in |
R707 |
T706 |
T703 |
arg2Of |
manner,in |
R708 |
T706 |
T704 |
arg1Of |
manner,an |
R709 |
T706 |
T705 |
arg1Of |
manner,identical |
R710 |
T693 |
T707 |
arg1Of |
expressed,to |
R711 |
T708 |
T707 |
arg2Of |
that,to |
R712 |
T708 |
T709 |
arg1Of |
that,which |
R713 |
T708 |
T710 |
arg1Of |
that,was |
R714 |
T711 |
T710 |
arg2Of |
used,was |
R715 |
T708 |
T711 |
arg2Of |
that,used |
R716 |
T713 |
T711 |
arg3Of |
obtain,used |
R717 |
T713 |
T712 |
arg1Of |
obtain,to |
R718 |
T708 |
T713 |
arg1Of |
that,obtain |
R719 |
T721 |
T713 |
arg2Of |
structure,obtain |
R720 |
T721 |
T714 |
arg1Of |
structure,the |
R721 |
T717 |
T715 |
arg1Of |
microscopy,high-resolution |
R722 |
T717 |
T716 |
arg1Of |
microscopy,cryo–electron |
R723 |
T721 |
T717 |
arg1Of |
structure,microscopy |
R724 |
T717 |
T718 |
arg1Of |
microscopy,( |
R725 |
T719 |
T718 |
arg2Of |
cryo-EM,( |
R726 |
T720 |
T718 |
arg3Of |
),( |
R727 |
T711 |
T722 |
arg1Of |
used,"," |
R728 |
T724 |
T723 |
arg1Of |
without,albeit |
R729 |
T711 |
T724 |
arg1Of |
used,without |
R730 |
T727 |
T724 |
arg2Of |
blockade,without |
R731 |
T727 |
T725 |
arg1Of |
blockade,a |
R732 |
T727 |
T726 |
arg1Of |
blockade,glycan-processing |
R733 |
T727 |
T728 |
arg1Of |
blockade,using |
R734 |
T729 |
T728 |
arg2Of |
kifunensine,using |
R735 |
T729 |
T730 |
arg1Of |
kifunensine,( |
R736 |
T731 |
T730 |
arg2Of |
4,( |
R737 |
T732 |
T730 |
arg3Of |
),( |
R738 |
T734 |
T733 |
arg1Of |
variant,This |
R739 |
T734 |
T735 |
arg1Of |
variant,of |
R740 |
T738 |
T735 |
arg2Of |
protein,of |
R741 |
T738 |
T736 |
arg1Of |
protein,the |
R742 |
T738 |
T737 |
arg1Of |
protein,S |
R743 |
T734 |
T739 |
arg1Of |
variant,contains |
R744 |
T742 |
T739 |
arg2Of |
glycans,contains |
R745 |
T741 |
T740 |
arg1Of |
22,all |
R746 |
T742 |
T741 |
arg1Of |
glycans,22 |
R747 |
T739 |
T743 |
arg1Of |
contains,on |
R748 |
T747 |
T743 |
arg2Of |
protein,on |
R749 |
T747 |
T744 |
arg1Of |
protein,the |
R750 |
T747 |
T745 |
arg1Of |
protein,SARS-CoV-2 |
R751 |
T747 |
T746 |
arg1Of |
protein,S |
R752 |
T747 |
T748 |
arg1Of |
protein,( |
R753 |
T750 |
T748 |
arg2Of |
1A,( |
R754 |
T751 |
T748 |
arg3Of |
),( |
R755 |
T750 |
T749 |
arg1Of |
1A,Fig. |
R756 |
T752 |
T753 |
arg1Of |
Stabilization,of |
R757 |
T757 |
T753 |
arg2Of |
structure,of |
R758 |
T757 |
T754 |
arg1Of |
structure,the |
R759 |
T757 |
T755 |
arg1Of |
structure,trimeric |
R760 |
T757 |
T756 |
arg1Of |
structure,prefusion |
R761 |
T752 |
T758 |
arg1Of |
Stabilization,was |
R762 |
T759 |
T758 |
arg2Of |
achieved,was |
R763 |
T752 |
T759 |
arg2Of |
Stabilization,achieved |
R764 |
T759 |
T760 |
arg1Of |
achieved,by |
R765 |
T761 |
T760 |
arg2Of |
using,by |
R766 |
T765 |
T761 |
arg2Of |
mutations,using |
R767 |
T765 |
T762 |
arg1Of |
mutations,the |
R768 |
T765 |
T763 |
arg1Of |
mutations,2P |
R769 |
T765 |
T764 |
arg1Of |
mutations,stabilizing |
R770 |
T765 |
T766 |
arg1Of |
mutations,( |
R771 |
T767 |
T766 |
arg2Of |
20,( |
R772 |
T768 |
T766 |
arg3Of |
),( |
R773 |
T761 |
T769 |
arg1Of |
using,at |
R774 |
T793 |
T769 |
arg2Of |
and,at |
R775 |
T772 |
T770 |
arg1Of |
and,residues |
R776 |
T771 |
T772 |
arg1Of |
986,and |
R777 |
T773 |
T772 |
arg2Of |
987,and |
R778 |
T772 |
T774 |
arg1Of |
and,"," |
R779 |
T780 |
T774 |
arg2Of |
substitution,"," |
R780 |
T780 |
T775 |
arg1Of |
substitution,a |
R781 |
T780 |
T776 |
arg1Of |
substitution,GSAS |
R782 |
T780 |
T777 |
arg1Of |
substitution,( |
R783 |
T778 |
T777 |
arg2Of |
Gly-Ser-Ala-Ser,( |
R784 |
T779 |
T777 |
arg3Of |
),( |
R785 |
T780 |
T781 |
arg1Of |
substitution,at |
R786 |
T785 |
T781 |
arg2Of |
site,at |
R787 |
T785 |
T782 |
arg1Of |
site,the |
R788 |
T785 |
T783 |
arg1Of |
site,furin |
R789 |
T785 |
T784 |
arg1Of |
site,cleavage |
R790 |
T785 |
T786 |
arg1Of |
site,( |
R791 |
T790 |
T786 |
arg2Of |
685,( |
R792 |
T791 |
T786 |
arg3Of |
),( |
R793 |
T790 |
T787 |
arg1Of |
685,residues |
R794 |
T790 |
T788 |
arg1Of |
685,682 |
R795 |
T790 |
T789 |
arg1Of |
685,to |
R796 |
T793 |
T792 |
arg1Of |
and,"," |
R797 |
T772 |
T793 |
arg1Of |
and,and |
R798 |
T797 |
T793 |
arg2Of |
motif,and |
R799 |
T797 |
T794 |
arg1Of |
motif,a |
R800 |
T797 |
T795 |
arg1Of |
motif,C-terminal |
R801 |
T797 |
T796 |
arg1Of |
motif,trimerization |
R802 |
T798 |
T799 |
arg1Of |
This,helps |
R803 |
T801 |
T799 |
arg2Of |
maintain,helps |
R804 |
T801 |
T800 |
arg1Of |
maintain,to |
R805 |
T798 |
T801 |
arg1Of |
This,maintain |
R806 |
T803 |
T801 |
arg2Of |
architecture,maintain |
R807 |
T803 |
T802 |
arg1Of |
architecture,quaternary |
R808 |
T801 |
T804 |
arg1Of |
maintain,during |
R809 |
T806 |
T804 |
arg2Of |
processing,during |
R810 |
T806 |
T805 |
arg1Of |
processing,glycan |
R811 |
T817 |
T807 |
arg1Of |
purified,Before |
R812 |
T808 |
T807 |
arg2Of |
analysis,Before |
R813 |
T817 |
T809 |
arg1Of |
purified,"," |
R814 |
T810 |
T811 |
arg1Of |
supernatant,containing |
R815 |
T815 |
T811 |
arg2Of |
S,containing |
R816 |
T815 |
T812 |
arg1Of |
S,the |
R817 |
T815 |
T813 |
arg1Of |
S,recombinant |
R818 |
T815 |
T814 |
arg1Of |
S,SARS-CoV-2 |
R819 |
T810 |
T816 |
arg1Of |
supernatant,was |
R820 |
T817 |
T816 |
arg2Of |
purified,was |
R821 |
T821 |
T817 |
arg1Of |
chromatography,purified |
R822 |
T810 |
T817 |
arg2Of |
supernatant,purified |
R823 |
T821 |
T818 |
arg2Of |
chromatography,by |
R824 |
T821 |
T819 |
arg1Of |
chromatography,size |
R825 |
T821 |
T820 |
arg1Of |
chromatography,exclusion |
R826 |
T823 |
T822 |
arg1Of |
ensure,to |
R827 |
T817 |
T822 |
modOf |
purified,to |
R828 |
T810 |
T823 |
arg1Of |
supernatant,ensure |
R829 |
T830 |
T823 |
arg2Of |
analyzed,ensure |
R830 |
T830 |
T824 |
arg1Of |
analyzed,that |
R831 |
T828 |
T825 |
arg1Of |
protein,only |
R832 |
T828 |
T826 |
arg1Of |
protein,native-like |
R833 |
T828 |
T827 |
arg1Of |
protein,trimeric |
R834 |
T828 |
T829 |
arg1Of |
protein,was |
R835 |
T830 |
T829 |
arg2Of |
analyzed,was |
R836 |
T828 |
T830 |
arg2Of |
protein,analyzed |
R837 |
T834 |
T830 |
arg3Of |
and,analyzed |
R838 |
T834 |
T831 |
arg1Of |
and,( |
R839 |
T834 |
T832 |
arg1Of |
and,Fig. |
R840 |
T833 |
T834 |
arg1Of |
1B,and |
R841 |
T835 |
T834 |
arg2Of |
fig,and |
R842 |
T836 |
T837 |
arg1Of |
S1,) |
R843 |
T840 |
T838 |
arg1Of |
conformation,The |
R844 |
T840 |
T839 |
arg1Of |
conformation,trimeric |
R845 |
T840 |
T841 |
arg1Of |
conformation,of |
R846 |
T844 |
T841 |
arg2Of |
material,of |
R847 |
T844 |
T842 |
arg1Of |
material,the |
R848 |
T844 |
T843 |
arg2Of |
material,purified |
R849 |
T840 |
T845 |
arg1Of |
conformation,was |
R850 |
T846 |
T845 |
arg2Of |
validated,was |
R851 |
T840 |
T846 |
arg2Of |
conformation,validated |
R852 |
T846 |
T847 |
arg1Of |
validated,by |
R853 |
T848 |
T847 |
arg2Of |
using,by |
R854 |
T850 |
T848 |
arg2Of |
EM,using |
R855 |
T850 |
T849 |
arg1Of |
EM,negative-stain |
R856 |
T850 |
T851 |
arg1Of |
EM,( |
R857 |
T853 |
T851 |
arg2Of |
1C,( |
R858 |
T854 |
T851 |
arg3Of |
),( |
R859 |
T853 |
T852 |
arg1Of |
1C,Fig. |