| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
0-103 |
DRI_Background |
denotes |
Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned. |
| T2 |
104-318 |
DRI_Background |
denotes |
Unexplained pneumonia (UP) caused by a novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in late December 2019 and has infected more than 9000 cases by 31 January 2020. |
| T3 |
319-419 |
DRI_Background |
denotes |
Shanghai reported the first imported case of COVID-19 (Coronavirus Disease 2019) in 20 January 2020. |
| T4 |
420-590 |
DRI_Background |
denotes |
A combinative approach of real-time RT-PCR, CRISPR-based assay and metagenomic next-generation sequencing (mNGS) were used to diagnose this unexplained pneumonia patient. |
| T5 |
591-654 |
DRI_Background |
denotes |
Real-time RT-PCR and CRISPR-based assay both reported positive. |
| T6 |
655-782 |
DRI_Approach |
denotes |
This sample belonged to Betacoronavirus and shared a more than 99% nucleotide (nt) identity with the Wuhan SARS-CoV-2 isolates. |
| T7 |
783-855 |
DRI_Outcome |
denotes |
We further compared pros and cons of common molecular diagnostics in UP. |
| T8 |
856-1053 |
DRI_Challenge |
denotes |
In this study, we illustrated the importance of combining molecular diagnostics to rule out common pathogens and performed mNGS to obtain unbiased potential pathogen result for the diagnosis of UP. |
