| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T1 |
51-176 |
DRI_Challenge |
denotes |
Severe acute respiratory syndrome coronavirus is the causative agent of a respiratory disease with a high case fatality rate. |
| T2 |
177-204 |
DRI_Background |
denotes |
During the formation of the |
| T3 |
243-412 |
DRI_Background |
denotes |
complex, essential steps include processing of the conserved polyprotein nsp7-10 region by the main protease Mpro and subsequent complex formation of the released nsp's. |
| T4 |
413-597 |
DRI_Approach |
denotes |
Here, we analyzed processing of the coronavirus nsp7-10 region using native mass spectrometry showing consumption of substrate, rise and fall of intermediate products and complexation. |
| T5 |
598-718 |
DRI_Background |
denotes |
Importantly, there is a clear order of cleavage efficiencies, which is influenced by the polyprotein tertiary structure. |
| T6 |
719-811 |
DRI_Approach |
denotes |
Furthermore, the predominant product is an nsp7+8(2 : 2) hetero-tetramer with nsp8 scaffold. |
| T7 |
812-989 |
DRI_Outcome |
denotes |
In conclusion, native MS, opposed to other methods, can expose the processing dynamics of viral polyproteins and the landscape of protein interactions in one set of experiments. |
| T8 |
990-1142 |
DRI_Outcome |
denotes |
Thereby, new insights into protein interactions, essential for generation of viral progeny, were provided, with relevance for development of antivirals. |
