LitCoin-sentences  

PubMed:28204828 JSONTXT 24 Projects

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Id Subject Object Predicate Lexical cue
T1 0-124 Sentence denotes TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway.
T2 125-344 Sentence denotes The transforming growth factor (TGF)-β-inducible early gene-1 (TIEG1) plays a crucial role in modulating cell apoptosis and proliferation in a number of diseases, including pancreatic cancer, leukaemia and osteoporosis.
T3 345-423 Sentence denotes However, the functional role of TIEG1 in the heart has not been fully defined.
T4 424-506 Sentence denotes In this study, we first investigated the role of TIEG1 in ischaemic heart disease.
T5 507-687 Sentence denotes For in vitro experiments, cardiomyocytes were isolated from both TIEG1 knockout (KO) and wile-type (WT) mice, and the apoptotic ratios were evaluated after a 48‑h ischaemic insult.
T6 688-782 Sentence denotes A cell proliferation assay was performed after 7 days of incubation under normoxic conditions.
T7 783-880 Sentence denotes In addition, the angiogenic capacity of endothelial cells was determined by tube formation assay.
T8 881-992 Sentence denotes For in vivo experiments, a model of myocardial infarction (MI) was established using both TIEG1 KO and WT mice.
T9 993-1111 Sentence denotes Echocardiography was performed at 3 and 28 days post-MI, whereas the haemodynamics test was performed 28 days post-MI.
T10 1112-1431 Sentence denotes Histological analyses of apoptosis, proliferation, angiogenesis and infarct zone assessments were performed using terminal deoxynucleotidyltransferase-mediated dUTP nick-end labelling (TUNEL) staining, BrdU immunostaining, α-smooth muscle actin (α-SMA)/CD31 immunostaining and Masson's trichrome staining, respectively.
T11 1432-1628 Sentence denotes Changes in the expression of related proteins caused by TIEG1 deficiency were confirmed using both reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis.
T12 1629-1840 Sentence denotes Our results demonstrated that the absence of TIEG1 prevented cardiomyocytes from undergoing apoptosis and promoted higher proliferation; it stimulated the proliferation of endothelial cells in vitro and in vivo.
T13 1841-2081 Sentence denotes Improved cardiac function and less scar formation were observed in TIEG1 KO mice, and we also observed the altered expression of phosphatase and tensin homolog (Pten), Akt and Bcl-2/Bax, as well as vascular endothelial growth factor (VEGF).
T14 2082-2245 Sentence denotes On the whole, our findings indicate that the absence of TIEG1 plays a cardioprotective role in ischaemic heart disease by promoting changes in Pten/Akt signalling.